骨疏颗粒对去卵巢骨质疏松大鼠骨代谢及子宫指数的影响  

Effect of Gushu Granule on Bone Metabolism and Uterus Index in Ovariectomized Osteoporosis Rats

作　　者：毛海琴[1,2] 杜义斌[1] 徐莹[1] 童晓云[1] 

机构地区：[1]云南中医学院第一附属医院,云南昆明650021 [2]广州中医药大学第一临床医学院,广东广州510405

出　　处：《新中医》2015年第2期213-215,共3页New Chinese Medicine

基　　金：云南省教育厅科学研究基金项目(2011Y410;2012C034);云南白药;云南中医学院中医药教育创新基金项目(YB2011J06);云南中医学院科研基金项目(XK201309);云南省中医医院院级科研项目(2011YJ034)

摘　　要：目的:观察骨疏颗粒对去卵巢骨质疏松大鼠骨代谢的影响,探讨骨疏颗粒防治骨质疏松的可能作用机制。方法:将3月龄SPF级SD雌性大鼠40只,随机分为假手术组、模型组、补佳乐(BJL)(戊酸雌二醇)组及骨疏颗粒(GSKL)组。假手术组只行假手术,其余3组去大鼠双侧卵巢复制绝经后骨质疏松症的模型。术后第4天开始分别给予对应药物灌胃,12周后检测血清钙、血清磷、尿钙与尿肌酐比值,称取子宫重量,计算子宫指数。结果:与假手术组比较,模型组尿钙与尿肌酐比值显著增加(P<0.05);与模型组比较,BJL组及GSKL组尿钙与尿肌酐比值显著降低(P<0.05)。各组血钙、血磷无明显变化(P>0.05)。与假手术组比较,模型组子宫指数显著降低(P<0.05);与模型组比较,BJL组子宫指数明显增加(P<0.05),GSKL组子宫指数略增加,差异无统计学意义(P>0.05)。结论:骨疏颗粒能降低骨吸收,桔抗骨质疏松。Objective： To observe the effect of Gushu Granule（GG） on bone metabolism in rats with ovariectomy-induced osteoporosis, and to explore its possible protective and therapeutic mechanism for osteoporosis. Methods- Forty SD female rats aged three-months were randomly divided into four groups, sham operation group, model group, Bujiale（BJL, estradiol valerate） group and GG group. The sham operation group only received operation for removing some fat, and the other three groups were given ovariectomy. Three days after ovariectomy, the rats were given corresponding drug by intragastric administration for twelve weeks. Serum levels of calcium and phosphorus, and ratio of urine calcium to urine creatinine were detected. The uterus was weighed for calculating uterus indexes. Results. The ratio of uterine calcium to uterine creatinine was increased in the model group（P〈 0.05 compared with the sham operation group）, and was decreased in BJL group and GG group （P 〈 0.05 compared with the model group）. No statistical significant differences of serum levels of calcium and phosphorus were found between the two groups（P 〉 0.05）. The uterus indexes was significantly decreased in the model group（P 〈 0.05 compared with the sham operation group）and then was obviously increased in BJL group（P 〈 0.05 compared with the model group） while was mildly increased in GG group（P〉 0.05）. Conclusion. Gushu granule can prevent osteoporosis by depressing bone absorption.

关 键 词：骨代谢 骨质疏松症 骨疏颗粒 大鼠 

分 类 号：R589.5[医药卫生—内分泌]