藻酸双酯钠对2型糖尿病db/db小鼠的糖脂代谢及糖尿病肾病的影响  

The effects of propylene glycol alginate sodium on hyperglycemic,hyperlipidemia and diabetic nephropathy in type 2 diabetic db/db mice

作　　者：周晓琳[1] 敦云楼 辛萌[1] 李鹏丽[1] 管华诗[1] 李春霞[1] 郝杰杰[1] 

机构地区：[1]中国海洋大学海洋药物教育部重点实验室,山东省糖科学与糖工程重点实验室,医药学院,山东青岛266003

出　　处：《中国海洋药物》2015年第1期40-46,共7页Chinese Journal of Marine Drugs

基　　金：教育部教师基金(20110132120021);青岛市科技发展技术(13-1-4-185-jch)资助

摘　　要：目的研究藻酸双酯钠(PSS)对2型糖尿病db/db小鼠模型高血糖、高血脂及糖尿病肾病的影响。方法取db/db小鼠随机分成5组(n=12),分别为模型对照组(db/db,蒸馏水)、阳性对照组(db/db,二甲双胍)、PSS低、中、高剂量组(db/db,PS25,PS50,PS100)以及正常对照组(C57/BL,蒸馏水),各组小鼠自由饮食进水90d。实验期间,每天记录进食量,每周记录体重,定期测定空腹血糖和糖化血红蛋白水平,末次给药后收集小鼠24h的尿液,用于检测尿白蛋白以及尿肌酐水平。实验结束后,测定小鼠血清中甘油三酯(TG)、低密度脂蛋白(LDL-c)、高密度脂蛋白(HDL-c)、总胆固醇(TC)含量。结果 PSS可以显著降低空腹血糖和糖化血红蛋白水平,提高胰岛素敏感性,并减少db/db小鼠的脂肪累积,显著改善db/db小鼠的血脂水平,缓解db/db小鼠糖尿病肾病症状。结论 PSS能够显著改善db/db小鼠的糖脂代谢紊乱以及糖尿病肾病症状。Objective To investigate the effects of propylene glycol alginate sodium （PSS） on hyperglycemic, hyperlipidemia and diabetic nephropathy in type 2 diabetic db/db mice. Methods The db/db mice were randomly divided into 5 groups （n= 12）, the model control group （db/db, distilled water）, the positive control group （db/db, Metformin）, the PSS low, middle and high doses groups （db/db, PS25, PS50, PS100） and the normal control group （C57/BL, distilled water）. The mice were fed with diet and water freely for 90 days. During the trial, the food intake was daily recorded, the body weight was measured weekly, fasting blood glucose levels and glycated hemoglobin levels were detected regularly. After the last administration, the mice urine was collected for 24h to detect the albumin and creatinine levels. At the end of experiment, the triglyceride （TG）, low-density lipoprotein （LDL-c）, high-density lipoprotein （HDL-c） and total cholesterol （TC） levels in serum were determined. Results PSS could significantly improve fasting glucose and glycated hemoglobin levels, ameliorate insulin resistance, relieve hyperlipidemia and alleviate the symptoms of diabetic nephropathy in db/db mice model. Conclusion PSS could effectively improve the lipid and glucose metabolism disorder and diabetic nephropathy.

关 键 词：藻酸双酯钠 DB/DB小鼠 2型糖尿病 

分 类 号：R965[医药卫生—药理学]