生长速度快且油脂产率高的湛江等鞭金藻诱变株的筛选  

Screening of fast growth and high oil productivity mutants of Isochrysis zhangjiangensis

作　　者：艾江宁[1] 姚长洪[1] 孟迎迎[1] 褚亚东[1] 曹旭鹏[1] 薛松[1] 

机构地区：[1]中国科学院大连化学物理研究所海洋生物工程组,辽宁大连116023

出　　处：《微生物学通报》2015年第1期142-147,共6页Microbiology China

基　　金：国家863计划项目(No.2012AA052101);辽宁省自然科学基金项目(No.2012010263);中化集团科技项目;中国科学院知识创新工程领域前沿项目(No.S201122)

摘　　要：【目的】筛选生长速度快的高产油湛江等鞭金藻诱变株。【方法】利用常压室温等离子体射流诱变技术对湛江等鞭金藻进行诱变,通过96孔板、摇瓶和反应器培养对诱变株进行筛选。【结果】传至第13代,600 m L反应器培养7 d,诱变株IM110020最大比生长速率、细胞密度和油脂产率可分别达0.72 d-1、16 750×104 cells/m L、109.8 mg/(L·d),分别比野生株提高12.5%、20.8%和17.9%。【结论】获得了一株生长速度快且油脂产率高的诱变株IM110020,且经过多次传代性质稳定。[Objective] Mutants of Isochrysis zhang/iangensis were screened for fast growth and high oil productivity. [Methods] Atmospheric and room temperature plasma jet mutation technique was used to mutate L zhangjiangensis, and mutants were screened by different cultivation volume such as 96-well plates, flasks and bioreactors culture. [Results] After thirteen times passaged, mutant IMl10020 was cultivated for 7 days in 600 mL bioreactor for evaluation. The maximum specific growth rate, cell density and lipid productivity could reach up to 0.72 d^-1, 16 750× 10^4 cells/mL and 109.8 mg/（L.d）, respectively, which were 12.5%, 20.8% and 17.9% better than those of the wild strain correspondingly. [Conclusion] We obtained a mutant IM110020 with fast growth and high oil productivity, and the properties were stable after thirteen passages.

关 键 词：常压室温等离子体 湛江等鞭金藻 生长速率 油脂产率 

分 类 号：Q949.2[生物学—植物学]