转基因小麦抗旱性鉴定及相关指标灰色关联度分析  

Characterization of drought resistance in transgenic wheat lines and grey correlation analyses of related traits

作　　者：罗影[1] 赵军[2] 王剑虹[3] 裴怀弟[4] 张斌科[5] 叶春雷[4] 李进京[4] 欧巧明[4] 王红梅[4] 王炜[4] 

机构地区：[1]甘肃农业大学资源与环境学院,甘肃兰州730070 [2]中国农业科学院生物技术研究所/国家农作物基因资源和基因改良重大科学工程,北京100081 [3]兰州职业技术学院生物工程系,甘肃兰州730070 [4]甘肃省农业科学院生物技术研究所,甘肃兰州730070 [5]甘肃农业大学农学院,甘肃兰州730070

出　　处：《干旱地区农业研究》2015年第1期48-53,共6页Agricultural Research in the Arid Areas

基　　金：国家转基因生物新品种培育重大专项(2009ZX08005-013B);甘肃省科技支撑项目(1104NKCA073);甘肃省农业科学院农业科技创新专项(2009GAAS15)

摘　　要：以9份转 ABP9基因春小麦株系UC4－1～UC4－9及其受体亲本宁春4号为材料，在雨养和灌溉条件下，对这些材料的主要农艺性状和部分生化指标进行了测定，采用隶属函数值法和抗旱性度量（D）值法对其抗旱性进行了综合评价，并利用灰色关联度法对相关抗旱性状与抗旱指数之间的关联度进行了分析评价。结果表明：6份转基因小麦的抗旱性较受体亲本宁春4号增强，3份材料的抗旱性较非转基因春小麦品种定西35增强；各农艺性状和生化指标与抗旱指数的关联度从高到低依次分别为株高、单穗粒重、穗长、SOD活性、穗粒数、结实小穗数、有效分蘖数、POD活性、千粒重、脯氨酸含量、MDA含量。A total of 11 traits ,including 7 agronomic traits and 4 biochemical traits ,was measured in nine ABP9-transgenic spring wheat lines and their donor parent Ningchun 4 that were grown in rain-fed （drought stress ） and well-wa-tered environments .Drought resistance capabilities of these materials were evaluated comprehensively by employing the methods of subordinate function and drought-resistance measure value （ D value ） . Correlation degrees between these traits and drought resistance indexes were analyzed and evaluated using grey relational grade analysis method .The results showed that the drought resistance of the 6 transgenic wheat lines was better than that of their donor parent Ningchun 4 . In particular ,3 lines even exhibited stronger drought resistance than a non-transgenic spring wheat cultivar Dingxi 35 , which was commonly used as a control for testing drought resistance of drought-land spring wheat in the northwest Chi-na .Results of grey relational grade analyses indicated that the correlation degrees between these traits and drought resis-tance indexes were ranked from high to low in the following order：plant height ,single spike weight ,spike length ,SOD activity ,number of kernel per spike ,effective tillers ,POD activity ,1000-kernel weight ,proline content ,and then MDA content .

关 键 词：转基因小麦 ABP9基因 抗旱性鉴定 

分 类 号：S512.1[农业科学—作物学] S332.4