小麦品种天选43抗条锈性遗传分析及抗病基因的分子标记  

Inheritance and molecular markers for yellow rust resistant gene( s) in wheat cultivar Tianxuan43

作　　者：姚未远[1] 柳泽光 樊玉[1] 申雪雪 巢凯翔[1] 候璐 王保通[1] 李强[1] 井金学[1] 李金昌[3] 

机构地区：[1]旱区作物逆境生物学国家重点实验室/西北农林科技大学植物保护学院,杨凌712100 [2]青海省农林科学院,西宁810016 [3]天水市农业科学研究所,天水741000

出　　处：《植物病理学报》2015年第1期48-56,共9页Acta Phytopathologica Sinica

基　　金：国家"863"课题(2012AA101503);国家"973"项目(2013CB127700);国家自然科学基金(31101396);陕西省科技统筹项目(2012KTCL02-10);高等学校学科创新引智计划(No.B07049)资助

摘　　要：天选43是由8845-01-01-1-1和抗源材料贵农22杂交选育而成的普通小麦品种,对我国目前所有条锈菌生理小种均表现良好抗性。为明确其抗条锈性遗传基础,本研究选用当前条锈菌流行小种CYR32和CYR33,对天选43与感病品种铭贤169杂交F1、F2和F3代群体进行遗传分析,同时应用460对SSR引物对接种CYR32的天选43/铭贤169 F2代150个单株群体进行抗病基因定位。结果表明,天选43对CYR32抗性由1对显性基因控制,而对CYR33抗性由1对隐性基因控制。筛选到10个与抗CYR32基因连锁的SSR标记Xwmc134、Xgwm413、Xbarc187、Xwmc406、Xcfd65、Xgwm18、Xbarc181、Xbarc137、Xwmc419和Xgwm230,两侧距离目的基因最近的标记为Xgwm18和Xgwm413,遗传距离分别为0.8 c M和3.4 c M,并初步将其抗病基因定位于小麦染色体1BS上,暂命名为Yr Tx43。基因来源、抗病遗传分析、分子标记检测及染色体位点分析表明,Yr Tx43很可能是与Yr24、Yr26具有等位性的抗条锈基因。Stripe rust, caused by Puccinia striiformis f.sp. tritici （Pst）, is one of the most devastating diseases of wheat throughout the world. Tianxuan 43, a wheat cultivar derived from hybridization between 8845-01-01-1- 1 and Guinong 22, is resistant to the current prevalent Pst races in China. To identify and map the gene （s） con- ferring resistance to stripe rust, Pst races including CYR32 and CYR33 were selected to test the F1, F2 and F3 generations derived from the cross Tianxuan 43/Mingxian 169 at seedling stage. SSR （simple sequence repeat） markers were used to map the gene conferring resistance to CYR32. The genetic analysis showed that one dominant resistance gene to CYR32 and one recessive resistance gene were estimated for Tianxuan 43. By using SSR to analyze the 150 individuals in the F2 segregating population, ten markers on chromosome 1BS including Xwmc134, Xgwm413, Xbarc187, Xwmc406, Xcfd65, Xgwml8, Xbarcl81, Xbarc137, Xwmc419 and Xgwm230, were identified to be linked with the resistance gene YrTx43 （temporarily designated）. The two closest linked flanking markers were Xgwml8 and Xgwm413 with genetic distances of 0.8 and 3.4 cM, respectively. According to origin, chromosome location and molecular detection, YrTx43 could be allelic to stripe rust resistance genes Yr24 and Yr26.

关 键 词：小麦条锈病 抗病基因 遗传分析 分子标记 

分 类 号：S435.12[农业科学—农业昆虫与害虫防治]