引起玉米粗缩病的水稻黑条矮缩病毒河南分离物的分子特征  

Molecular characterization of Rice black-streaked dwarf virus isolates causing maize rough dwarf disease in Henan

作　　者：刘瑞丽[1] 袁媛[1] 王振跃[1] 王英志[1] 袁虹霞[1] 张晓婷[1] 施艳[1] 李洪连[1] 

机构地区：[1]河南农业大学植物保护学院植物病理学系,郑州450002

出　　处：《植物病理学报》2015年第1期101-105,共5页Acta Phytopathologica Sinica

基　　金：国家"十二五"粮食丰产科技工程项目(2012BAD04B07);河南省现代农业产业技术体系专项(S2010-02-G05);中国科学院知识创新重大项目(KSCX2-EW-N-08)

摘　　要：目前已报道的造成玉米粗缩病的病原有3种，分别是玉米粗缩病毒 （ Maize rough dwarf virus, MRDV），水稻黑条矮缩病毒（ Rice black-streaked dwarf virus, RBSDV ） 和南方水稻黑条矮缩病毒（Southern rice black-streaked dwarf virus, SRBS- DV）。我国于1954年在新疆和甘肃发现该病，Rice black-streaked dwarf virus （RBSDV） is the main pathogen that causes maize rough dwarf, rice black-streaked dwarf and wheat green dwarf in China, leading to severe damage of crop yield and quality. In this study RBSDV infected maize leaves were collected from eight areas in Henan province. RT-PCR was conducted to amplify the full-length s10 gene for sequencing. The results showed that the s10 of all the eight isolates were 1 801 bp in length which encoded a protein of 558 amino acids （aa）. The nucleotide and amino acid homology among the eight isolates were 93.0%-99.88% and 98.20% ~ 99.80%, respectively, indicating that the variation of nucleotides was more obvious than that of amino acids.

关 键 词：水稻黑条矮缩病毒 玉米粗缩病毒 分子特征 分离物 virus 河南 ROUGH 病原 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治]