“双固一通”电针法对老年阳虚证模型大鼠肝线粒体结构及ATP酶活性的影响  

Effect of “Double Reinforcing and One Unblocking” Electroacupuncture on Hepatic Mitochondrial Structure and ATPase Activity in a Rat Model of Senile Yang Deficiency

作　　者：刘建民[1] 吕景芳[2] 王华[1] 刘静[3] 陈明扬[4] 王静芝[1] 

机构地区：[1]湖北中医药大学,武汉430061 [2]十堰市妇幼保健院,十堰442000 [3]孝感市中心医院,孝感432000 [4]武汉市中医医院,武汉430010

出　　处：《上海针灸杂志》2015年第1期63-66,共4页Shanghai Journal of Acupuncture and Moxibustion

基　　金：湖北省自然科学基金重点项目(2008CDA051)

摘　　要：目的探讨"双固一通"电针法对老年阳虚证模型大鼠肝线粒体形态结构及ATP酶活性的影响。方法选用5月龄SD雄性大鼠40只,随机分为A组、B组、C组和D组,每组10只。A组为正常对照组,其余3组大鼠均皮下注射D-半乳糖40 d后再肌肉注射氢化可的松7 d制作老年阳虚证大鼠模型。C组采用"双固一通"电针法,电针关元、足三里、百会穴;D组采用电针中极、阴陵泉、印堂穴治疗。每星期治疗6次,共治疗4星期。治疗后以游泳力竭时间判定各组大鼠抗疲劳能力,并采用透射电镜观察肝线粒体结构、比色法检测各组大鼠肝组织Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶活性。结果与A组比较,B组和D组大鼠平均游泳力竭时间明显缩短(均P<0.01),肝线粒体内外膜模糊不清,嵴变形、断裂或消失,肝线粒体出现肿胀等改变,Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶活性均下降(P<0.01,P<0.05)。C组比B组游泳力竭时间明显延长(P<0.01),肝线粒体形态结构得到明显改善,Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶活性均明显提高(P<0.01,P<0.05);D组与B组比较,大鼠平均游泳力竭时间缩短(P<0.05),肝粗面内质网有脱颗粒,肝Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶活性降低(P<0.05)。结论 "双固一通"电针法可改善老年阳虚证模型大鼠阳虚抗疲劳能力,改善肝线粒体超微结构,提高肝Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶活性,促进肝脏能量代谢。Objective To explore the effect of ＂double reinforcing and one unblocking＂ electroacupuncture on hepatic mitochondrial structure and ATPase activity in a rat model of senile yang deficiency. Methods Forty 5-month-old male SD rats were selected and randomly allocated to groups A, B, C and D, 10 rats each. Group A was a normal control group. A model of senile yang deficiency was made by 40 days＇ subdermal injection of D-galactose and then 7 days＇ intramuscular injection of hydrocortisone in the other three groups of rats. Group C received treatment with ＂double reinforcing and one unblocking＂ electroacupuncture at points Guangyuan（CV4）, Zusanli（ST36） and Baihui（GV20）; group D, treatment with electroacupuncture at points Zhongji（CV3）, Yirdingquan（SP9） and Yintang（GV29）. Treatment was given 6 times a week for a total of 4 weeks. After treatment, the anti-fatigue ability was determined by swimming exhaustion time, hepatic mitochondrial structure was observed under a transmission electron microscope and hepatic Na^＋-K^＋-ATPase and Ca^2＋-Mg^2＋-ATPase activities were measured by colorimetry in every group of rats. Results The mean swimming exhaustion time shortened significantly （all P〈0.01）; hepatic mitochondrial inner and outer membranes were blurred, mitochondrial ridges deformed, ruptured or disappeared and mitochondria swelled; both Na^＋-K^＋-ATPase and Ca^2＋-Mg^2＋-ATPase activities decreased （P〈0.01, P〈0.05） in groups B, C and D of rats compared with group A. Swimming exhaustion time lengthened significantly （P〈0.01）, hepatic mitochondrial structure improved significantly and both Na^＋-K^＋-ATPase and Ca^2＋-Mg^2＋-ATPase activities increased significantly （P〈0.01, P〈0.05） in group C compared with group B. Rat mean swimming exhaustion time shortened （P〈 0.05）, hepatic rough endoplasmic reticula degranulated and hepatic Na^＋-K^＋-ATPase and Ca^2＋-Mg^2＋-ATPase activities decreased （P〈0.05） in group D compared with g

关 键 词：针刺疗法 电针 阳虚证 肝线粒体 ATP酶 “双固一通”电针法 大鼠 

分 类 号：R2-03[医药卫生—中医学]