电针内关穴预处理对兔心肌缺血再灌注CK及心肌细胞活性的影响  

Effect of Pretreatment with Electroacupuncture at Point Neiguan(PC6) on CK and Myocardial Cell Activity in Rabbit Myocardial Ischemia and Reperfusion

作　　者：王超[1] 张佳丽[1] 刘昭[1] 刘密[1] 严洁[1] 常小荣[1] 郭礼娜 艾坤[1] 

机构地区：[1]湖南中医药大学,长沙410007

出　　处：《上海针灸杂志》2015年第1期67-69,共3页Shanghai Journal of Acupuncture and Moxibustion

基　　金：国家自然科学基金项目(81072868;81102661);湖南省科技厅课题(2013sk3086)

摘　　要：目的观察电针内关穴预处理对心肌缺血再灌注损伤兔CK及心肌细胞活性的影响,为证实心包经穴位与心之间的相对特异性联系提供实验依据。方法将40只新西兰大耳白兔随机分为A组、B组、C组、D组和E组,每组8只。A组为假手术组;B组为缺血再灌注模型组,于左冠状动脉前降支阻断冠脉30 min,再灌注60 min;C组、D组和E组在造模前7 d,每日分别取内关、列缺及合谷给予电针刺激20 min。比较各组肌酸激酶(CK)及心肌formazan含量变化。结果 B组、C组、D组和E组血清CK及心肌formazan含量与A组比较,差异均具有统计学意义(P<0.01,P<0.05)。C组、D组和E组血清CK含量与B组比较,差异均具有统计学意义(P<0.01)。C组心肌formazan含量与B组比较,差异具有统计学意义(P<0.05)。D组和E组血清CK及心肌formazan含量与C组比较,差异均具有统计学意义(P<0.01,P<0.05)。结论电针内关穴能减轻心肌缺血再灌注对心肌细胞的损伤,从而对心脏产生保护作用。Objective To investigate the effect of pretreatment with electroacupuncture at point Neiguan（PC6） on CK and myocardial cell activity in rabbit myocardial ischemia-reperfusion injury and provide an experimental basis for a relatively specific relationship between pericardium meridian points and the heart. Methods Forty New Zealand big ear rabbits were randomly allocated to groups A, B, C, D and E, eight rabbits each. Group A was a sham operation group. Group B was an ischemia-reperfusion model group, in which the left anterior descending coronary artery was occlude for 30 min and reperfused for 60 min. In groups C, D and E, points Neiguan（PC6）, Lieque（LU7） and Hegu（LI4） were separately given electroacuptmcture stimulation, 20 min every day, at seven days before model making. Creatine kinase （CK） and myocardial formazan contents were compared between the groups. Results There were statistically significant differences in serum CK and myocardial formazan contents between group B, C, D or E and group A （P〈0.01, P^0.05）. There was a statistically significant differences in serum CK content between group C, D or E and group B （P〈0.01）. There was a statistically significant differences in myocardial formazan content between groups C and B （P〈0.05）. There were statistically significant differences in serum CK and myocardial formazan contents between group D or E and group C （P〈0.01, P〈0.05）. Conclusion Electroacupunc^re at point Neiguan（PC6） can reduce myocardial ischemia-reperfusion induced injury to myocardial cells to produce a protective effect on the heart.

关 键 词：针刺疗法 电针 心肌再灌注损伤 肌酸激酶 心肌细胞活性 穴 内关 穴 列缺 穴 合谷 兔 

分 类 号：R2-03[医药卫生—中医学]