基于纳米RuO_x的微型电化学胰岛素传感器  

Micro-Fabricated Electrochemical Insulin Sensor Modified with RuO_x Nano Particles

作　　者：王君[1] 周洁[1] 许迎科[1] 王慷慨[2] 王菊[2] 刘涌[2] 刘清君[1] 王平[1] 

机构地区：[1]浙江大学生物传感器国家专业实验室,杭州310027 [2]浙江大学硅材料国家重点实验室,杭州310027

出　　处：《电子科技大学学报》2015年第1期155-160,共6页Journal of University of Electronic Science and Technology of China

基　　金：国家自然科学基金(61320106002);中国博士后基金(2014M561764);浙江省博士后科研项目择优资助(BSH1402030)

摘　　要：采用MEMS工艺及C-MEMS工艺制备碳微电极,在其上修饰纳米氧化钌Ru Ox颗粒后,表现出对胰岛素的电催化氧化特性,可实现胰岛素的电化学检测。以此构建的微型电化学胰岛素传感器,具有较好的电化学性能,其检测灵敏度为1 n A/μM,检测限为800 n M。该传感器结合流动注射分析系统,可获得稳定的测量,且所需样品量少,易实现长时、连续检测,具有潜在的临床应用价值,也可构建细胞传感器,在胰腺β细胞分泌胰岛素的机理研究中具有良好的应用前景。Insulin detection is significant in clinical study, but the lack of effective detection methods impedes its clinical applications. In this work, the carbon microelectrode fabricated with MEMS （micro-electromechanical system） and C-MEMS （complex micro-electromechanical system） technology is applied for insulin detection. The carbon microelectrodes were fabricated through the pyrolysis of photoresist and showed good electrochemical performance. After electro-deposition of ruthinium oxide nano particles on the electrode surface, the insulin oxidation is electro-catalyzed and its electrochemical detection is realized at 0.7 V. The sensor has a sensitivity of 1 nA/μM for insulin detection, with a detection limit of 800 nM. Electrochemical measurement shows that the sensor has advantages of high sensitivity, fast response and usability for real time on-site detection.

关 键 词：碳微电极 电化学生物传感器 胰岛素检测 纳米金属氧化物 

分 类 号：TH77[机械工程—仪器科学与技术]