百蕊草提取物对免疫球蛋白A肾病模型大鼠的治疗作用  

Therapeutic effects of Thesium chinensis extracts on IgAN rats

作　　者：闫俊 唐大海 宣伟东 范正平 胡水根 

机构地区：[1]解放军411医院药学科,上海200081 [2]江西中医学院药学院,江西南昌333004

出　　处：《药学实践杂志》2015年第1期28-31,35,共5页Journal of Pharmaceutical Practice

基　　金：上海市卫生局局级科研课题(2012085)

摘　　要：目的考察百蕊草提取物对免疫球蛋白A肾病(IgAN)模型大鼠的治疗效果,以明确有效部位。方法采用水煎提取百蕊草,AB-8大孔树脂吸附,乙醇梯度洗脱制备各部位。给予大鼠口服牛血清蛋白(400 mg/kg),皮下注射蓖麻油、CCl4联合尾静脉注射脂多糖造模。从第8周起,对阳性组[雷公藤多苷(LGT),10mg/kg]、水提取物组(TT,1.0g/kg)、水溶性部位组(TTW,0.4g/kg)、极性部位组(TT20,0.4g/kg)和中等极性部位组大鼠(TT50,0.4g/kg)每天灌胃给药,空白组和模型组给予生理盐水(n=8),连续5周。观察各组大鼠24h尿量、24h尿蛋白、尿沉渣红细胞计数,实验结束后进行血液生化指标检测、免疫荧光检测、肾组织的病理学检查。结果与空白组相比,模型组大鼠的24h尿蛋白、尿沉渣中红细胞数量、血清中肌酐、尿素氮水平均显著升高。与模型组相比,TT组和TT50组的24h尿蛋白(P<0.05)、尿沉渣中红细胞数量(P<0.01)、血清肌酐和尿素氮水平(P<0.05)降低。各给药组肾组织系膜区的免疫复合物沉积减少、肾小球增大、系膜基质增生、肾小管的损伤状况改善。结论百蕊草水提物及其中等极性部位对IgA肾病大鼠具有良好的治疗作用,其效果优于阳性对照雷公藤多苷。Objective To evaluate the therapeutic effects of Thesium chinensis Turcz extracts on IgA nephropathy （IgAN） rats .Methods The hot water extracted solution of Thesium chinensis Turcz flow through the AB8 macroreticular resin column repeatedly ,then being eluted with different contents of ethanol to get four part of extract .The IgAN model of SD rats were established by oral intake of bovine serum albumin（BSA ,400 mg/kg） together with injection of lipopolysaccharide（LPS） and CCl4 .Beginning with 8th week ,the positive group（LGT ,10 mg/kg） ,water extract group（TT ,1 .0 g/kg） ,water soluble group（TTW ,0 .4 g/kg） ,high polarity group（TT20 ,0 .4 g/kg） and medium polarity group（TT50 ,0 .4 g/kg） rats were intragastric administrated drug daily ,the normal group control and model control group rats were given with saline（n=8） .During the next 5 weeks treatment process ,the 24 h urine volume ,24 h urine protein concentration ,and quantity of erythrocyte in urine were observed .The levels of TP ,ALP ,ALT ,AST ,Scr and BUN in serum were determined .The expression of IgA deposition was measured by immunofluorescence staining ,and the pathological changes of kidney tissue were assayed after the therapy process .Results After 5 weeks therapy ,the 24 h urine protein concentration ,the quantity of erythrocyte in urine ,and the level of Scr and BUN of TT and TT50 groups were significantly lower than model control .The fluorescence intensities in the mesangial area were weaker than model control .Also ,the degrees of renal capsule expansion and mesangial matrix proliferations , and the kidney tubules damage of TT and TT50 groups were weaker than those of model control .Conclusion The water extract and its medium polarity fraction of Thesium chinensis Turcz show good therapeutic effects on IgAN rats .

关 键 词：百蕊草 免疫球蛋白A肾病 SD大鼠 中药治疗 

分 类 号：R965[医药卫生—药理学]