木犀草素对脂多糖诱导下小鼠急性肺损伤的保护作用及其机制研究  

Study on protective effects and its imechanism of luteolin on acute lung injury induced by lipopolysaccharide in mice

作　　者：戴丽[1] 端莉梅[1] 姚志清[1] 

机构地区：[1]南京大学医学院附属口腔医院,南京市210008

出　　处：《河北医药》2015年第3期339-342,共4页Hebei Medical Journal

摘　　要：目的探讨木犀草素(luteolin)对脂多糖(1ipopolysaccharid,LPS)诱导的小鼠急性肺损伤的保护作用,以及可能的作用机制。方法 96只小鼠随机分为正常组、模型组、木犀草素低剂量组、木犀草素中剂量组、木犀草素高剂量组及地塞米松组,每组8只。以小鼠气道穿刺滴入LPS制备小鼠ALI模型,呼吸机检测气道吸气阻力(Ri)、气道呼气阻力(Re)和动态肺顺应性(Cdyn)的变化,ELISA法测定肺泡灌洗液(BALF)中IL-1β、IL-6和TNF-α的浓度,制作HE病理切片以及Western blot法检测肺组织中NF-κB p65、IκB-α和PIκB-α的表达情况。结果木犀草素能明显抑制Ri、Re增长和Cdyn降低,抑制BALF中IL-1β、IL-6、TNF-α的释放(P<0.05),减轻了肺部病变,抑制了NF-κB p65和PIκB-α的活化。结论木犀草素对LPS诱导的小鼠急性肺损伤具有保护作用,其作用机制与抑制NF-κB信号传导通路表达有关。Objective To investigate the effect of luteolin on acute lung injury （ ALI） induced by lipopolysaccharide （LPS） in mice,and to explore possible action mechanism .Methods Ninety-six mice were randomly divided into 6 groups：notmal control group ,model group ,luteolin low-dose group luteolin median-dose group luteolin high-dose group ,dexamethasone control group ,with 8 mice in each group .The animal models with ALI were established by injecting LPS into airway of mice , then the changes of lung airway inspiratory resistance （Ri）,expiratory resistance （Re） and dynamic lung compliance （Cdyn） were detected by pulmonary function test apparatus .The levels of IL-1β,IL-6,TNF-αin bronchoalveolar lavage fluid （ BALF） were determined by ELISA .The pathological sections were made ,and the histopathological changes were observed under light microscope.The expression levels of NF-κB p65,IκB-αand PIκB-αin lung tissues were detected by Western blotting .Results Luteolin could obviously inhibit Ri ,Re and reduce Cdyn ,and could significantly decrease the concentrations of IL-1β,IL-6 and TNF-αin BALF （ P 〈0.05）,as a result,which could relieve lung injury ,moreover,luteolin could inhibit the activation of NF-κB p65 and PIκB-α.Conclusion Luteolin has a protective effect on ALI induced by LPS in mice , and its action mechanism is correlated to inhibiting the expression of NF-κB signal transduction pathway .

关 键 词：木犀草素 急性肺损伤 脂多糖 肺功能 

分 类 号：R563[医药卫生—呼吸系统]