Rh部分D基因型分析  

Analysis for partial RhD genotype

作　　者：何路军[1] 乔芳[1] 石翠英[1] 王振雷[1] 赵志弘[1] 刘敬闪[1] 张虹[1] 田亚娟[1] 

机构地区：[1]河北省血液中心,石家庄市050071

出　　处：《河北医药》2015年第2期179-181,共3页Hebei Medical Journal

基　　金：河北省科学技术研究与发展计划(编号:09276102D-40)

摘　　要：目的研究7例Rh部分D样本的基因分型。方法采用PCR-SSP技术检测常规血清学试验为D变异型的样本中的Rh部分D进行D基因分型。结果在30例常规血清学试验中,23例具有完整的RHD基因外显子(Rh弱D型),7例部分外显子缺失,为Rh部分D,23例为弱D型,并对部分D样本进行了基因分型,4例检测为D Cat.ⅥⅢ,1例为D Cat.Ⅵtype2,1例为D Cat.Ⅵtype 1,1例为基因2.9外显子融合,表示为RHD-CE(2-9)-D。结论 Rh部分D型与弱D型在血清学试验中无法区分,采用分子生物学方法才能够更准确的分型,更能确保输血安全。Objective To investigate genotyping of 7 cases of partial RhD specimens .Methods The genetyping technique （ PCR-SSP ） was performed for partial RhD variants identified by common serological tests .Results Among 30 specimens identified as D variants during routine serological experiment ,7 specimens were defined as partial RhD and 23 specimens as weak D .Then 7 specimens of partial RhD were genetyped further in which 4 cases were D Cat .ⅥⅢ,1 case was D Cat.Ⅵ type 2,1cases was D Cat.Ⅵ type 1 and 1 case was RHD-CE（2-9）-D.Conclusion The routine serological experiments can not differentiate partial D from weak D ,however ,the molecular biological methods can type accurately in order to ensure the safety of blood transfusion .

关 键 词：RH阴性 Rh部分D 弱D 基因型 

分 类 号：R349.6[医药卫生—基础医学]