一种用于细菌药物敏感试验的快速纸片扩散法的建立  

A rapid Kirby-Bauer method in antimicrobial susceptibility test

作　　者：王恒秋 刘雨峰 王双 胡志方 

机构地区：[1]韶关市中医院检验科,广东韶关512031 [2]韶关市中医院内科三区,广东韶关512031

出　　处：《国际检验医学杂志》2015年第2期165-167,共3页International Journal of Laboratory Medicine

基　　金：2014年韶关市科技计划(医学类)项目(2014CX/Ka07)

摘　　要：目的建立一种快速纸片扩散（K-B）法用于细菌药物敏感试验（AST）。方法快速K-B法是在低倍镜下快速观测抑菌环直径并获得AST结果的K-B法,快速K-B法和K-B法不同在于在不同时间点检测细菌抑菌环直径,比较两种方法对金黄色葡萄球菌、其他葡萄球菌、大肠埃希菌、肠杆菌科其他细菌、铜绿假单胞菌和其他非发酵菌的抑菌环直径平均检出时间,各观测时间抑菌环直径检出率。计算快速K-B法、K-B法与标准K-B法的符合率并进行比较。结果快速K-B法与K-B法对6种细菌（菌龄相同）抑菌环直径平均检出时间比较差异均有统计学意义（P〈0.05）。两种方法对大于或等于72h菌龄的6种细菌抑菌环直径平均检出时间均大于菌龄小于或等于48h的细菌,差异有统计学意义（P〈0.05）。同种方法对6种小于或等于48h菌龄的不同细菌抑菌环直径平均检出时间比较差异均有统计学意义（P〈0.05）,同种方法对6种大于或等于72h菌龄的不同细菌抑菌环直径平均检出时间比较差异均无统计学意义（P〉0.05）。快速K-B法与标准K-B法对抑菌环直径检测的符合率为88.89%~100.00%,K-B法与标准K-B法符合率为100.00%。结论快速K-B法获得AST时间比自动细菌鉴定仪和标准K-B法均快,标准K-B法进行AST,无需等待18~24h观测抑菌环直径,只要肉眼可见抑菌环,即可测试其直径而获得AST结果。Objective To establish a rapid method of Kirby-Bauer（K-B） for antimicrobial susceptibility test （AST）. Methods Rapid K-B method was a K-B method observing bacteriostatic ring diameter at low magnification. K-B and K-B method were used to detect the bacteriostatic ring diameter at different time point. The average checkout time of Staphylococcus aureus,other Staphylo- coccus aureus, Escherichia coli,other Escherichia coli, Pseudomonas aeruginosa and other bacteria fermentation, the detection rate of of bacteriostatic ring diameter at different observation time, the coincidence rates of standard K-B method, and rapid K-B method were compared. Results The average checkout time of two methods on 6 kinds of bacteria bacteriostatic ring diameter （448 h and ≥72 h） had statistically significant differences（P〈0.05）. The average checkout time of the bacteria younger than 48 hours were significant higher than those of bacteria older than 72 h（P〈0.05）. The average checkout time of the different bacteria with age younger than 48 h by same method had significant difference（P〈0.05）. The average checkout time of the different bacteria with age older than 72 h by same method had no significant difference（P〉0.05）. Compared with the standard K-B method,the co- incidence rate of rapid K-B method was 88.89%- 100.00%, that of K-B method was 100.00%. Conclusion Rapid K-B method was faster on obtaining the AST than the standard K-B method and automatic bacteria identification. Standard K-B method for AST,should not wait for 18--24 h to observe bacteriostatic ring diameter,as long as it is visible to the naked eyes could test its di- ameter and get AST results.

关 键 词：快速纸片扩散法 抑菌环 药物敏感试验 

分 类 号：R446.5[医药卫生—诊断学]