4-氨基安替比林分光光度法测定苯酚滴耳液中苯酚含量  

Determination of phenol in phenol ear drops by 4-amino-antipyrine spectrophotometry

作　　者：潘杰[1] 戴学文[2] 李晗[2] 房志仲[2] 

机构地区：[1]天津市中心妇产科医院药剂科,天津300100 [2]天津医科大学药学院药剂学教研室,天津市临床药物关键技术重点实验室,天津300070

出　　处：《天津医科大学学报》2015年第1期87-89,共3页Journal of Tianjin Medical University

摘　　要：目的:探讨一种更为简便、快速的测定苯酚滴耳液中苯酚含量的方法。方法:苯酚与4-氨基安替比林在铁氰化钾的作用下,溶液显橙红色,在520 nm处呈最大吸收,可直接用分光光度法测定苯酚的含量。结果:在520 nm测定波长下,苯酚浓度在1.0、1.5、2.0、2.5、3.0、3.5、4.0和4.5μg/m L范围内呈良好的线性关系。回归方程:Y=0.1408X-4.4762×10-3(r=0.999 9,n=3),测定方法的平均回收率为99.72%,平均日内差和日间差RSD分别为0.44%和0.63%;3批样品的含量较为均匀,平均含量为0.196 g/m L,平均RSD为0.63%。结论:该方法较为简便,灵敏,结果准确可靠。Objective： To develop a simple, effective method for content determination of phenol in phenol ear drops. Methods： Under the effect of potassium ferricyanide, the solution containing phenol and 4-aminoantipyrene showed orange red and the maximum absorption wavelength was 520 nm. The contents was directly determined by spectrophotometric method. Results： With the maximum absorption wavelength being 520 nm, the linear range of phenol was 1.0-4.5 μg/mL （r=0.999 9, n=3）, and the regression equation was Y=0.1408X- 4.4762×10^-3. The average recovery rate was 99.72%, and RSD of intra-day and inter-day precision were 0.44% and 0.63%, respectively. No significant difference was found in the contents of the samples determined by established method, with the average content being 0.196 g/mL and RSD 0.63%. Conclusion：The established method is simple, efficient and accurate.

关 键 词：苯酚 滴耳液 4-氨基安替比林 分光光度法 含量测定 

分 类 号：R927.2[医药卫生—药学]