热预处理对超负荷训练大鼠心肌蛋白激酶C表达的影响  

Effects of Heat Preconditioning on Expression of Protein Kinase C in Myocardium of Rats after Overload Exercise

作　　者：杨翼[1] 李章华 石武谛[1] 柳华[1] 张杰[1] 

机构地区：[1]武汉体育学院健康科学学院,湖北武汉市430079 [2]武汉市第三医院,湖北武汉市430060

出　　处：《中国康复理论与实践》2015年第1期49-53,共5页Chinese Journal of Rehabilitation Theory and Practice

基　　金：国家自然科学基金项目(No.30700388);湖北省自然科学基金一般项目(No.2011CDB313);湖北省教育厅科研计划重点项目(No.D20144101)

摘　　要：目的观察热预处理(HP)对超负荷训练大鼠心肌蛋白激酶C(PKC)δ、PKCε表达的影响。方法 3月龄雄性Sprague-Dawley大鼠25只分为对照组(n=5)、训练组(n=10)和HP组(n=10)。超负荷训练8周后应用免疫组化法、Real-time PCR和Western blotting检测心肌组织PKCδ、PKCε表达。结果三种检测方法均显示,训练组大鼠心肌PKCδ表达较对照组和HP组增高(P<0.05)。Real-time PCR与免疫组化检测结果显示,训练组PKCε表达低于对照组和HP组(P<0.05);Western blotting结果显示三组PKCε表达无显著性差异(P>0.05)。结论在热预处理心肌保护效应中,PKC可能发挥了重要的作用。Objective To investigate the effects of heat preconditioning （HP） on the expression of protein kinase C （PKC）δand PKCεin the myocardium of overload exercise rats. Methods 25 male three-month-old Sprague-Dauley rats were divided into control group （n=5）, exercise group （n=10） and HP group （n=10）. The expression of PKCδand PKCεin the myocardium was detected with immunohistochemis-try, real-time PCR and Western blotting 8 weeks after overload exercise. Results The expression of PKCδsignificantly increased in the exer-cise group compared with the control group and the HP group （P〈0.05）. In another hand, PCR and immunohistochemistry showed the ex-pression of PKCεin the exercise group decreased compared with the control group and the HP group （P〈0.05）, but it was not significantly different among the groups using Western blotting （P〉0.05）. Conclusion PKCs may play an important role in the HP-induced cardio-protec-tion during overload exercise.

关 键 词：热预处理 心肌 蛋白激酶C 过度运动 大鼠 

分 类 号：R873[医药卫生—运动医学]