一种骨骼细胞动态压缩载荷发生装置的设计  

Design of dynamic compression load generator for bone cells

作　　者：刘戈[1] 罗二平[1] 申广浩[1] 王天博[2] 翟明明[1] 佟世超[1] 景达[1] 

机构地区：[1]第四军医大学生物医学工程学院,西安710032 [2]大连理工大学交通运输学院,辽宁大连116024

出　　处：《医疗卫生装备》2015年第1期1-4,共4页Chinese Medical Equipment Journal

基　　金：陕西省自然科学基金(2014JQ4139);国家自然科学基金(31270889)

摘　　要：目的:设计一种小型、便携且简单可控的体外细胞压应力加载装置,能够用于骨骼细胞力学信号转导的实验研究。方法:首先,利用Solid Works进行装置的外观图绘制,采用聚甲基丙烯酸甲酯材料设计该加载装置,该材料具有良好的热传导性能;其次,通过基于个人计算机(personal computer,PC)端的Lab VIEW软件控制单元控制运动控制卡驱动电动机驱动器,保证电动机获得精确的运动控制;最后,通过数据采集卡在基于PC端的Lab VIEW采集单元实时采集压力传感器输出的压力信号,并进行数据的实时显示和存储。结果:该小型化装置在实验中能够置于细胞培养箱中,从而可以保证在适宜的细胞生长环境中进行力学信号转导实验,提高实验的稳定性和可重复性;该装置具有良好的工作稳定性,通过Lab VIEW控制端程序,可以实现对伺服电动机动作的精确控制和对应力数据的精确采集。结论:该空气动态载荷发生装置具有小型、便携等优势,对于骨骼细胞力学信号转导的实验研究具有广阔的应用前景。Objective To design a mechanical loading device with air pressure, which is employed for the investigation of in vitro skeletal cellular mechanotransduction. Methods Firstly, the three-dimensional drawing for the structure of the device was performed using Solid Works software, and the mechanical loading chamber was enclosed with the polymethyl methacrylate, which possessed excellent thermo-conduction property. Then, the PC-based Lab VIEW control unit was programmed to drive the motor controller to make sure that the motion of the motor was controlled accurately. Finally, the pressure signals acquired by the sensor were sampled at real time by the PC-based Lab View program through the DAQ card. Results The mechanical loading device with air pressure was able to fit the size of the regular incubator for cell culture, and thus provided a favorable living environment for the in vitro cell growth, which might greatly increase the stability and repeatability for the in vitro experiment for cell mechanotransduction. Moreover, this device also exhibited excellent working stability. The motion of the actuator could be precisely controlled, and the signals of temperature and loads could be accurately acquired. Conclusion The present mechanical loading system has unique advantages, such as the miniaturization and portability, etc, which may shed light for the study of mechanotranduction study and possess bright application potential for the future mechanotranduction investigation in bone cells.

关 键 词：应力加载装置 细胞信号转导 伺服电动机 压力传感器 LAB VIEW 

分 类 号：R318.01[医药卫生—生物医学工程] R318.6[医药卫生—基础医学]