表观遗传调控滇重楼内生真菌活性物质的合成  

Epigenetic Manipulation Regulates Antimicrobial Components Synthesis of Endophytic Fungi from Paris polyphylla var. yunnanensis

作　　者：姜上川[1] 

机构地区：[1]清华大学生命科学学院,北京100084

出　　处：《北京联合大学学报》2015年第1期19-25,共7页Journal of Beijing Union University

基　　金：青年科学基金项目(20121302384)

摘　　要：提出化学表观筛选——分子遗传平台策略,利用表观遗传理论调控滇重楼内生真菌抗菌活性物质的合成。化学表观遗传筛选是对真菌进行高通量化学表观抑制剂的筛选,旨在经济快捷地获得抗菌活性物质合成受到表观调控的菌株;建立分子遗传平台是根据同源基因克隆真菌的相关表观遗传酶类基因,靶向改变表观遗传酶类的表达,旨在获得遗传稳定性高的改良菌株。Here we put forward a strategy called chemical epigenetic screening and molecular genetics platform, which applies epigenetic theory on regulating antimicrobial components synthesis of endophytic fungi from Paris polyphylla var. yunnanensis. Chemical epigenetic screening is the high-through screening of fungi via different epigenetic inhibitors to efficiently and economically acquire the strains with significantly altered components synthesis. While molecular genetics platform can lay the foundation for cloning genes encoding specific enzymes involving in epigenetic regulation and interrupting the target enzyme expression level, so as to obtain genetically improved strains.

关 键 词：表观遗传调控 内生真菌 抗菌活性物质 化学表观筛选 分子遗传平台 

分 类 号：Q75[生物学—分子生物学]