孕期碘营养状态对子代大鼠成骨细胞的影响  

Effects of iodine on osteoblasts in pup rats

作　　者：马红梅[1] 宫建[2] 郭凤[3] 杨传家[4] 于嵩[5] 李福生[6] 郑刚[7] 李斯文[7] 

机构地区：[1]中国医科大学附属口腔医院材料教研室,辽宁沈阳110001 [2]沈阳药科大学生命科学与生物制药学院临床药学教研室,辽宁沈阳110016 [3]中国医科大学药学院药物毒理学教研室,辽宁沈阳110001 [4]中国医科大学附属盛京医院胰腺甲状腺外科,辽宁沈阳110001 [5]辽宁中医药大学解剖组胚教研室,辽宁沈阳110847 [6]辽宁省人民医院骨肿瘤科,辽宁沈阳110016 [7]沈阳医学院奉天医院骨外三科,辽宁沈阳110024

出　　处：《口腔医学》2014年第12期885-888,955,共5页Stomatology

基　　金：国家自然科学基金(81200765);高等学校博士学科点专项科研基金(20122104120020)

摘　　要：目的观察孕期碘营养状态的改变对大鼠仔鼠成骨细胞的的影响。方法雌性Wistar大鼠,交配妊娠后,取孕鼠20只按体质量随机分成对照Ⅰ组、对照Ⅱ组、碘缺乏组、碘过量组。自妊娠日起,对照Ⅰ组饲以普通饲料+去离子水,对照Ⅱ组饲以缺碘饲料+适碘去离子水,碘缺乏组饲以缺碘饲料+去离子水,碘过量组饲以普通饲料+高碘水。孕18日时,检测各组孕鼠甲状腺激素和促甲状腺激素水平;仔鼠出生后,取出生24 h内仔鼠的颅骨成骨细胞进行培养,观察成骨细胞碱性磷酸酶活性和细胞增殖能力。结果碘缺乏组甲状腺激素含量较对照Ⅰ组、对照Ⅱ组和碘过量组明显降低(均P<0.05),促甲状腺激素含量则明显升高(均P<0.05);碘过量组甲状腺激素含量与对照Ⅰ组和对照Ⅱ组间差别没有统计学意义(均P>0.05),但促甲状腺激素含量则明显升高(均P<0.05)。碘缺乏组成骨细胞碱性磷酸酶活性和细胞增殖能力较对照Ⅰ组、对照Ⅱ组和碘过量组明显下降(均P<0.05)。结论大鼠孕期碘营养状态不足,可导致新生仔鼠成骨细胞活性和增殖能力下降。Objective To study the effects ofiodine onosteoblasts in pup rats. Methods Female Wistar rats（n = 20） after pregnan- cy were randomly divided into four groups：control group Ⅰ ,control group Ⅱ ,iodine deficient group,iodine overdose group. The control group I was fed with a normal diet and drank deionized water,while the control group H was fed with an iodine-deficient diet and drank deionized water with adequate iodine during the experiment. The iodine deficient group was fed with an iodine-deficient diet and drank deionized water during the experiment,while the iodine overdose group was fed with a normal diet and drank deionized water with high iodine. On gestational day 18, the levels of thyroid hormones and thyroid stimulating hormone were tested in pregnant rats. On post- natal day 0, alkalinephosphatase activities and proliferation of osteoblasts were observed in pup rats. Results The levels of thyroid hor- mones in iodine deficient group were significant lower than the controls （ all P 〈 0.05 ） , while the levels of thyroid stimulating hormone increased significantly（ all P 〈 0.05）. There were no significant differences in the levels of thyroid hormones between the iodine over- dose group and the controls（ all P 〉 0.05 ）. However,a significant up-regulation of thyroid stimulating hormone was observed in the io- dine overdose group compared to the controls （ all P 〈 0.05 ）. The alkalinephosphatase activities and proliferation of osteoblasts were sig- nificantly down-regulated in iodine deficient group compared to other groups（ all P 〈 0.05 ）. Conclusions Iodine deficiency in preg- nant rats led to down-regulation of alkalinephosphatase activities and proliferation of osteoblasts inpup rats.

关 键 词：碘 甲状腺激素 促甲状腺激素 成骨细胞 牙齿 大鼠 

分 类 号：R348.5[医药卫生—基础医学]