桑菊饮对内毒素诱导小鼠急性肺损伤的保护作用  

Sangjuyin attenuates acute lung injury induced by lipopolysaccharide in mice

作　　者：张天柱[1,2] 杨世海[2] 张景龙[1] 

机构地区：[1]长春中医药大学,长春130117 [2]吉林农业大学,长春130117

出　　处：《中药药理与临床》2014年第5期12-14,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：吉林省科学技术厅科技引导计划项目(200905110)

摘　　要：目的:探讨桑菊饮对内毒素(lipopolysaccharide,LPS)诱导小鼠的急性肺损伤(acute lung injury,ALI)保护作用。方法:于小鼠腹腔注射LPS(5 mg/kg)复制ALI动物模型。将小鼠随机分为空白组对照组、LPS组、地塞米松组(2 mg/kg)、桑菊饮水提物组(6.5g/kg、13g/kg)。观察各组肺组织病理学改变,测量肺湿/干重比,支气管肺泡灌洗液中性粒细胞百分比、肺组织中超氧化物歧化酶(SOD)、丙二醛(MDA)、炎症因子白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)含量及肺组织中p38MAPK与NFκ-BP65蛋白表达。结果:桑菊饮组(6.5 g/kg、13g/kg)可有效减轻LPS所致肺组织病理学变化,能降低肺泡灌洗液中炎症细胞的数目,能显著降低肺湿/干重比重,能提高SOD水平,并能降低MDA水平,减低p38MAPK与NFκBP65蛋白。结论:桑菊饮6.5 g/kg、13g/kg可减轻LPS所致急性肺组织损伤,对LPS诱导的急性肺损伤有保护作用。Objective： To determine the protective effect of Sangjuyin water extract on acute lung injury induced by lipopolysaccharide（ LPS）.Methods： The BABL / C mice were randomly allocated into control,LPS,dexamethasone（ 2mg / kg） 、Sangjuyin water extracts（ 6. 5 g / kg,13 g / kg）. The bronchoalveolar lavage fluid（ BALF） samples were analyzed for the concentration of numbers of neutrophilic,superoxide dismutase（ SOD）,methane dicarboxylic aldehyde（ MDA）,interleukin-6（ IL-6） and tumor necrosis factor alpha（ TNF-α）,and The lung samples were taken for histologic evaluation and for determination of wet-to-dry（ W / D） lung weight,The protein levels of p38 MAPK and NFκ-BP65 were detected by Western blot analysis. Results Pretreatment with Sangjuyin water extract（ 6. 5 g / kg,13 g / kg） prior to the administration of intratracheal LPS significantly induced a decrease in lung wet weight / dry weight ratio,in total leukocyte number and neutrophil percent in the BALF,and in MDA activity of lung in dose-dependent manners,and in IL-6,the tumor neurosis factor-α（ TNF-α） in the BALF and p38 MAPK and NFκ-BP65 protein in lung. These results showed that anti-inflammatory effects of Sangjuyin water extract（ 6. 5 g / kg,13 g / kg）against the LPS-induced ALI.

关 键 词：桑菊饮 LPS-诱导急性肺损伤 P38MAPK NFκBP65 

分 类 号：R285[医药卫生—中药学]