胡椒碱对胆汁反流性胃炎大鼠胃粘膜的作用研究  

Effect and mechanism of piperine (the main component of Radix Piperis longi) on gastric mucosa in bile reflux gastritis rats

作　　者：卢婷[1] 高鸿亮[1] 席利力[1] 罗兰[2] 吕顺忠[2] 刘发[3] 姚萍[1] 

机构地区：[1]新疆医科大学第一附属医院消化科,乌鲁木齐830054 [2]新疆医科大学第一附属医院药学部,乌鲁木齐830054 [3]新疆医科大学药学院药理学研究室,乌鲁木齐830054

出　　处：《中药药理与临床》2014年第5期26-29,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金(81260670)

摘　　要：目的:探讨维药荜茇根主要成分胡椒碱对胆汁反流性胃炎模型大鼠胃粘膜的作用及其机制。方法:将60只大鼠随机分为正常组、模型组、胡椒碱42.2mg/kg剂量组、胡椒碱21.1mg/kg剂量组、胡椒碱10.5mg/kg剂量组及多潘立酮组共6组。除正常组使用蒸馏水灌胃,其余采用自制反流液给予大鼠灌胃建立实验性胆汁反流性胃炎模型。连续造模7日后药物干预,每日造模后6小时给予药物治疗,造模并药物治疗35日后,使用放射免疫法测定血清胃泌素(GAS)含量,免疫组化法测定胃粘膜中cerb-b2、p21及PCNA蛋白表达量,RT-PCR方法测定胃粘膜COX-2mRNA表达含量。结果:胡椒碱42.2mg/kg、21.1mg/kg、10.5mg/kg剂量组大鼠胃粘膜的炎细胞浸润程度低于模型组。胡椒碱21.1mg/kg、10.5mg/kg剂量组及多潘立酮组大鼠血清GAS含量高于模型组。胡椒碱42.2mg/kg、21.1mg/kg剂量组及多潘立酮组大鼠胃粘膜P21阳性表达量高于模型组。胡椒碱42.2mg/kg、21.1mg/kg、10.5mg/kg剂量组COX-2mRNA表达量低于模型组。结论:荜茇的主要成分胡椒碱对胆汁反流性胃炎模型大鼠胃粘膜具有保护作用,荜茇根主要成分胡椒碱可提高胆汁反流性胃炎模型大鼠血清胃泌素、胃粘膜P21蛋白表达含量并降低COX-2mRNA表达含量。Objective： To investigate the effect and mechanism of piperine（ the main component of Radix Piperis longi） on gastric mucosa in bile reflux gastritis rats. Methods： The each half of the 60 male and female SD rats were randomly divided into 6 groups： Normal group,model group,high dose of piperine group,middle dose of piperine group,low dose of piperine group,domperidone group. Use distilled water in addition to the normal group,and the remaining groups were gavaged with homemade reflux liquid in order to establish the experimental BRG model. After 7 days modeling continuous,then give drug treatment six hours after modeling a day,the experiment lasts 35 days. Serum gastrin（ GAS） was determined by radioimmunoassay,measuring cerb-b2,p21 and PCNA protein expression by immunohistochemistry,RT-PCR method for the determination of gastric mucosal expression of COX-2mRNA content. Results： High,middle and low dose of piperine group of rat with gastric mucosal inflammatory cell infiltration are lesser than the model group（ P 〈 0. 01）. Compared with the model group,the content of rat serum GAS of middle、low dose of piperine group are higher（ P 〈 0. 05）. The P21 expression in rat gastric mucosa of high 、middle dose of piperine group and domperidone group are higher than the model group（ P 〈 0. 05）. The high,middle and low dose of piperine group expression of COX-2mRNA are lower than the model group（ P 〈 0. 01）. Conclusion： Piperine which is the main ingredient of Radix Piperis longi can protect the bile reflux gastritis model rat gastric mucosa. The main component of Uygur medicine Radix Piperis longi that piperine can increase serum gastrin、gastric P21 protein content and reduce expression of COX-2mRNA content of bile reflux gastritis rats.

关 键 词：胡椒碱 荜茇根 胆汁反流性胃炎 GAS P21 COX-2MRNA 

分 类 号：R285[医药卫生—中药学]