The Achyranthes bidentata polypeptide k fraction enhances neuronal growth in vitro and promotes peripheral nerve regeneration after crush injury in vivo  

作　　者：Qiong Cheng Chunyi Jiang Caiping Wang Shu Yu Qi Zhang Xiaosong Gu Fei Ding 

机构地区：[1]Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregenration, Nantong University

出　　处：《Neural Regeneration Research》2014年第24期2142-2150,共9页中国神经再生研究（英文版）

基　　金：supported by a grant from National Key Basic Research Program of China(973 Program),No.2014CB542202;a grant from Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)in China

摘　　要：We have previously shown that Achyranthes bidentata polypeptides （ABPP）, isolated from Achyranthes bidentata Blume （a medicinal herb）, exhibit neurotrophic and neuroprotective effects on the nervous system. To identify the major active component of ABPP, and thus optimize the use of ABPP, we used reverse-phase high performance liquid chromatography to separate ABPP. We obtained 12 fractions, among which the fraction of ABPPk demonstrated the strongest neuroactivity. Immunocytochemistry and western blot analysis showed that ABPPk promoted neurite growth in cultured dorsal root ganglion explant and dorsal root ganglion neurons, which might be associated with activation of Erk1/2. A combination of behavioral tests, electrophysiological assessment, and histomorphometric analysis indicated that ABPPk enhanced nerve regeneration and function restoration in a mouse model of crushed sciatic nerve. All the results suggest that ABPPk, as the key component of ABPP, can be used for peripheral nerve repair to yield better outcomes than ABPP.We have previously shown that Achyranthes bidentata polypeptides （ABPP）, isolated from Achyranthes bidentata Blume （a medicinal herb）, exhibit neurotrophic and neuroprotective effects on the nervous system. To identify the major active component of ABPP, and thus optimize the use of ABPP, we used reverse-phase high performance liquid chromatography to separate ABPP. We obtained 12 fractions, among which the fraction of ABPPk demonstrated the strongest neuroactivity. Immunocytochemistry and western blot analysis showed that ABPPk promoted neurite growth in cultured dorsal root ganglion explant and dorsal root ganglion neurons, which might be associated with activation of Erk1/2. A combination of behavioral tests, electrophysiological assessment, and histomorphometric analysis indicated that ABPPk enhanced nerve regeneration and function restoration in a mouse model of crushed sciatic nerve. All the results suggest that ABPPk, as the key component of ABPP, can be used for peripheral nerve repair to yield better outcomes than ABPP.

关 键 词：nerve regeneration Achyranthes bidentata polypeptides neuroactive component dorsal root ganglion neurite outgrowth crush injury sciatic nerve peripheral nerve regeneration neural regeneration 

分 类 号：R651.3[医药卫生—外科学]