免疫胶体金标记定位昆诺藜中的胼胝质和胼胝质降解酶  

Study on the distribution of callose and β-1,3-D-glucanase in Chenopodium quinoa by immuno-gold labeling

作　　者：李云琴[1] 王华[1] 谢礼 洪健[1] 

机构地区：[1]浙江大学农生环测试中心,浙江杭州310058 [2]浙江省农业科学研究院病毒学与生物技术研究所,浙江杭州310021

出　　处：《电子显微学报》2014年第6期558-563,共6页Journal of Chinese Electron Microscopy Society

基　　金：国家"211工程"项目"高等学校仪器设备和优质资源共享系统"资助(项目号:CERS-1-29)

摘　　要：运用免疫胶体金技术研究了感染蚕豆萎蔫病毒2号以及健康昆诺藜细胞中胼胝质和胼胝质降解酶的分布情况。电镜观察发现胶体金特异性标记在含有VP37小管的胞间运动通道周围,其余区域感病和健康细胞的胼胝质及胼胝质降解酶分布差别不大,胼胝质主要分布于细胞间隙的大块电子致密物质以及细胞壁结构发生较大变化的区域,胼胝质降解酶主要分布于筛板、维管束细胞紧贴细胞壁区域的大块电子致密物质上,在细胞壁结构发生较大改变的区域也有胼胝质降解酶的分布。推测胼胝质在昆诺藜细胞中的积累和降解可能与细胞壁结构成分改变有关。The distribution of callose and β-1,3-D-glucanase in the Broad bean wilt virus 2 （ BBWV 2 ） infected and the healthy Chenopodium quinoa was investigated by using immuno-gold labeling with the antibody against callose and β-1, 3-D-glucanase, respectively. The results show that, except for the VP37 intercellular moving cell wall pathways, there is no obvious difference on the distribution of callose and β-1,3-D-glucanase between the infected and healthy cells. Callose is mainly distributed on the regions as bellow：the electron condensed material which located in the intercellular space, the structural altered region of cell wall and the cell wall broken region. Glucanase is mainly distributed on the regions as bellow：the sieve plate, the electron condensed material attached to cell wall in the vascular bundle and the structural altered region of cell wall. From these results, it can be deduced that the accumulation and degradation of callose in C. quinoa is related to the cell wall structural and component alteration.

关 键 词：β-1  3-D-葡聚糖(胼胝质) 胼胝质降解酶 蚕豆萎蔫病毒2号 昆诺藜 免疫胶体金标记 

分 类 号：S432.2[农业科学—植物病理学]