人工麝香水提物对脂多糖诱导RAW264.7细胞炎性介质表达的影响  

Effect of Artificial Musk Aqueous Extract on the Expressions of Inflammatory Mediators Released from Lipopolysaccharide-stimulated RAW264. 7 Cells

作　　者：孟迂 邹秦文 白金叶[2] 程桂芳[2] 肖宣[2] 章菽 朱秀媛[2] 王晓良[2] 

机构地区：[1]北京联馨药业有限公司技术部,北京102628 [2]中国医学科学院北京协和医学院药物研究所北京市重点实验室,北京100050

出　　处：《中国医学科学院学报》2014年第6期583-586,共4页Acta Academiae Medicinae Sinicae

摘　　要：目的研究人工麝香水提物对脂多糖(LPS)刺激小鼠单核巨噬细胞RAW264.7释放炎症因子的影响,为建立体外评价模型奠定基础。方法采用酶联免疫吸附法测定细胞上清液中白介素(IL)-6、IL-10和肿瘤坏死因子α的含量。结果不同剂量的人工麝香水提物可明显降低LPS引起的细胞分泌IL-6、IL-10和肿瘤坏死因子α的含量。结论在LPS刺激单核巨噬细胞RAW264.7释放炎症因子模型中,人工麝香水提物可明显降低炎性因子的释放,可能是其抗炎作用的主要机制之一。Objective on lipopolysaccharide-stimulated cytokines secreted or released by RAW264. 7 cells. Methods Cytokines inclu- ding intedeukin （IL） -6, IL-10, and tumor necrosis factor α were determined using cytokine enzyme-linked immunosorbent assay kits. Result Compared with model group, the levels of major cytokines such as tumor necrosis factor α, IL-6, and IL-10 significantly decreased in different AME groups in a dose-dependent manner. Conclusion In lipopolysaccharide-stimulated RAW264. 7 macrophages, AME can remarkably inhibit the re- lease of inflammatory cytokines and thus exerts its anti-inflammatory effects.

关 键 词：人工麝香 白介素-6 白介素-10 肿瘤坏死因子α 

分 类 号：R967[医药卫生—药理学]