大鼠深Ⅱ°烫伤创面组织中血管内皮生长因子的表达变化  

Expression of Vascular Endothelial Growth Factor in Deep Second-degree Scald Wounds in Rats

作　　者：吴起[1] 王甲汉[1] 李志清[1] 任加良[1] 吴永恒[1] 

机构地区：[1]南方医科大学南方医院烧伤科,广州510515

出　　处：《中国医学科学院学报》2014年第6期650-653,共4页Acta Academiae Medicinae Sinicae

摘　　要：目的观察大鼠深Ⅱ°烫伤创面愈合过程中血管内皮生长因子(VEGF)的表达变化,进一步探讨VEGF对烫伤创面愈合的影响。方法将36只Wistar大鼠完全随机分为烫伤组(30只)及对照组(6只),烫伤组制作深Ⅱ°烫伤模型,于伤后第1、3、7、14、21天各处死6只大鼠,取创面全层组织,通过实时荧光定量PCR和Western blot分别检测创面组织中VEGF的mRNA含量和蛋白表达水平。对照组不予烫伤,直接处死,取正常皮肤检测VEGF的mRNA含量和蛋白表达水平。结果烫伤组大鼠创面组织中VEGF的mRNA含量及蛋白表达水平于伤后显著增加,1 d即达峰值,3 d逐渐减少,14 d为最低值,21 d又再次增加;与对照组比较,差异有统计学意义(P<0.05)。结论 VEGF参与调节烫伤创面的修复过程,VEGF在烫伤创面愈合过程中的表达变化与创面血管形成过程密切相关。Objective To observe the expression of vascular endothelial growth factor （VEGF） in deep second-degree scald wounds, with an attempt to further explore the role of VEGF in burn wound healing. Methods Totally 36 adult Wistar rats were randomized into two groups： the scald group （30 rats） and the control group （6 rats） . In the scald group, rat models of deep second-degree scald wounds were established. Full-thickness tissues of the wounds were collected respectively 1, 3, 7, 14, and 21 days after the modeling. The expressions of the VEGF mRNA and protein were detected with real-time quantitative PCR and Western blot, respectively. In the control group, the same procedures were performed but without modeling. Results Com- pared with the control group, the expressions of VEGF mRNA and Proteins were significantly higher in the scald group （P 〈 0. 05） . The expression levels reached the peak on day 1, gradually decreased on day 3, reached the lowest points on day 14, but increased again on day 21. Conclusions VEGF is involved in the healing of scald bums. The expression of VEGF during the wound healing is closely correlated with the wound angiogenesis.

关 键 词：血管内皮生长因子 烫伤 创面 修复 

分 类 号：R644[医药卫生—外科学]