中药三子排痰汤联合氨溴索对哮喘大鼠气道黏液高分泌的影响  

Effects of the traditional Chinese medicine San Zi Pai Tan Tang combined with ambroxol in the treatment of airway mucus hypersecretion in rats with asthma

作　　者：吴云涛[1] 霍博雅[2] 

机构地区：[1]辽宁医学院研究生院,辽宁锦州121001 [2]辽宁医学院附属第三医院中医科,辽宁锦州121001

出　　处：《解放军医学杂志》2014年第12期956-960,共5页Medical Journal of Chinese People's Liberation Army

摘　　要：目的研究三子排痰汤联合氨溴索(Ambroxol)对哮喘大鼠气道黏液高分泌的影响作用。方法 40只SD大鼠按随机数字表法分为5组:空白组、哮喘组、三子排痰汤组(中药组)、氨溴索组(西药组)、三子排痰汤联合氨溴索组(中西结合组),每组8只。采用卵清白蛋白(OVA)复制大鼠哮喘模型,于实验第42、56d对大鼠进行形态学评分;采用苏木素-伊红(HE)染色观察气道炎症;免疫组化法检测肺组织黏蛋白(MUC5AC)表达;酶联免疫法(EILSA)测定血中白细胞介素13(IL-13)含量;过碘酸雪夫氏(PAS)染色法检测气道杯状细胞增生。结果所有药物干预组的形态学评分与气道炎症均较哮喘组明显减轻;哮喘组肺组织MUC5AC表达量高于空白组(0.530±0.035 vs 0.132±0.020,P<0.05),中西结合组、中药组、西药组MUC5AC表达量均低于哮喘组(P<0.05),且中西结合组与中药组、西药组的差异均有统计学意义(P<0.05);中西结合组、中药组和西药组血中IL-13含量均较哮喘组明显减少(42.41±3.15、46.31±3.07、43.05±2.70 vs 51.65±4.07,P<0.05),且中西结合组与中药组的差异有统计学意义(P<0.05),而与西药组差异无统计学意义;中西结合组和中药组、西药组气道杯状细胞增生面积均较哮喘组明显减少(41.21±2.88、48.10±3.44、51.06±3.51 vs 63.25±5.02,P<0.05),且中西结合组与中药组、西药组的差异均有统计学意义(P<0.05)。结论三子排痰汤联合氨溴索能抑制哮喘大鼠气道黏液的过度分泌,其机制可能是通过调节IL-13和杯状细胞实现的,而且其疗效优于单纯的中药或西药治疗。Objective To investigate the effects of the traditional Chinese medicine SanZiPaiTanTang combined with ambroxol in the treatment of airway mucus hypersecretion in rats with asthma. Methods Forty SD rats were randomly divided into 5 groups （8 each）： normal group, asthma group, SanZiPaiTanTang group （Chinese medicine group）, ambroxol group （Western medicine group）, SanZiPaiTanTang combined with ambroxol group （The combinational group）. The rat asthma model was reproduced with ovalbumin （OVA）, and all these rats were morphologically scored on the 42th and 56th day. Airway inflammation was observed pathologically with HE staining, and the expression of lung tissue mucin （MUC5AC） was examined by immunohistochemistry. The IL-13 content in serum was measured by enzyme-linked immunosorbent assay （ELISA）, and the proliferation of goblet cells in airway was detected by periodic acid Schiff （PAS） staining. Results The morphological score and airway inflammation decreased in all drug treated groups as compared with those in asthma group. The MUC5AC levels were higher in asthma group than in normal group （0.530±0.035 vs 0.132±0.020, P〈0.05）, the combination group （0.254±0.029）, the Chinese medicine group （0.359±0.025） and the Western medicine group （0.386±0.030） （P〈0.05）, while the difference between combinational group and Chinese medicine group or Western medicine group was statistically significant （P〈0.05）. The IL-13 contents markedly decreased in the combinational group, Chinese medicine group and Western medicine group compared with that in asthma group （42.41±3.15, 46.31±3.07, 43.05±2.70 vs 51.65±4.07, P〈0.05）, and the difference between the combinational group and Chinese medicine group was statistically significant （P〈0.05）, but no significant di？erence was found between the combinational group and the Western medicine group. The goblet cell hyperplasia area was significantly lower in the three drug treated groups than in asthma gr

关 键 词：支气管哮喘 气管 体内分泌物 三子排痰汤 氨溴索 黏蛋白类 

分 类 号：R289.5[医药卫生—方剂学]