不同剂量60 Co γ射线照射对大鼠颌下腺凋亡的影响  

Effects of different doses of 60Co γ-ray irradiation on apoptosis of rat submandibular gland cells

作　　者：韦富贵[1] 孙文忠[1] 

机构地区：[1]柳州市人民医院耳鼻咽喉科,广西壮族自治区柳州545000

出　　处：《中国医师杂志》2014年第12期1630-1633,共4页Journal of Chinese Physician

基　　金：广西科学基金资助项目（桂科自0728241）

摘　　要：目的 探讨不同剂量60Coγ射线照射对大鼠颌下腺凋亡及相关因子表达的影响.方法 将Wistar大鼠随机分4组：（1）正常对照组;（2）放疗7.5Gy组;（3）放疗15 Gy组;（4）放疗22.5 Gy组.放疗组大鼠给予一次性γ射线辐射,每只辐射量按分组设定的剂量（7.5、15、22.5 Cy）给予.对照组未予照射.采用免疫组织化学法检测P53、Caspase-3表达情况以及原位切口末端标记（TUNEL）法检测细胞凋亡.结果 与对照组比较,放疗组P53与Caspase-3的表达增强.Tunel染色显示的细胞凋亡主要见于导管细胞,随着放疗剂量增加,凋亡愈明显.结论 60Co γ射线照射可引起大鼠颌下腺早期细胞凋亡.60Co γ射线（7.5、15、22.5 Gy）照射诱导的颌下腺细胞凋亡有剂量-效应关系.Objective To explore effects of different doses of 60Co γ-ray irradiation on apoptosis of rat submandibular gland dells.Methods Wistar rats were randomly divided into four different radiotherapy dosage groups （0 G y,7.5 G y,15 Gy,and 22.5 Gy）.Rats in each radiotherapy groups were irradiated by the required dose of γ-ray irradiation （0 Gy,7.5 Gy,15 Gy,22.5 Gy） at one time.Immunohistochemistry method and TdT-mediated dUTP nick end labeling （TUNEL） methods were used to detect expression of P53,caspase-3,and apoptosis of submandibular gland dells in rats.Results The expression levels of P53 and caspase-3 of rat submandibular cells were significantly increased in different radiotherapy dosage groups （7.5 G y,15 Gy,and 22.5 Gy） relative the 0 Gy dosage group.The apoptotic cells were more commonly seen in duct cells in different radiotherapy dosage groups,and the apoptotic cells were gradually increased with the increased radiotherapy dosages.Conclusions 60 Co γ-ray irradiation could lead to apoptosis of rat submandibular gland in the early stage.There is a dose-effect relationship between 60Co γ-ray irradiation and induced apoptosis of rat submandibular gland cells.

关 键 词：γ射线/治疗应用 辐射损伤 颌下腺 细胞凋亡 

分 类 号：R817[医药卫生—影像医学与核医学]