补肾通络方对rIL-1β诱导的大鼠肾小球系膜细胞增殖及TGF-β_1、CTGF的影响  

The Effects of Bushentongluo Fomula on Rat Glomerular Mesangial Cells Proliferation and Expression of TGF- β_1 and CTGF Induced by rIL-1β

作　　者：刘湘丹[1] 袁林祥 卢岳华[2] 周日宝[1] 童巧珍[1] 

机构地区：[1]湖南中医药大学,长沙410208 [2]湖南师范大学,长沙410081

出　　处：《中国中西医结合肾病杂志》2014年第12期1047-1051,共5页Chinese Journal of Integrated Traditional and Western Nephrology

基　　金：湖南省教育厅/财政厅重点项目(No.12A098);湖南省教育厅大学生创新项目(No.2013-176);湖南中医药大学优秀教师培养项目(2013)

摘　　要：目的:研究补肾通络方含药血浆对重组大鼠白细胞介素-1β(r IL-1β)干预的大鼠肾小球系膜细胞(GMCs)增殖与TGF-β1和CTGF表达的影响,以探讨补肾通络方抗肾脏纤维化的分子作用机制。方法:体外培养GMCs,制备补肾通络方含药血浆,用白细胞介素对GMCs进行干预,MTT比色法检测GMCs增殖、ELISA检测TGF-β1蛋白表达,RT-PCR检测CTGF mRNA的表达。结果:与空白对照组比较,白细胞介素能诱导GMCs的增殖、促TGF-β1生成、上调CTGF表达;补肾通络方含药血浆能下调由白细胞介素介导的GMCs增殖、TGF-β1蛋白和CTGF mRNA的表达量,与白细胞介素组比差异有统计学意义(P<0.01),且呈时间依赖性。结论:补肾通络方抗肾脏纤维化作用与抑制TGF-β1和CTGF的表达,调节GMCs的功能密切相关。Objective：To investigate the effect of BuShenTongLuo Fomula（ BSTLF）on cell proliferation,the protein expres-sion of TGF-β1 and the mRNA expression of CTGF in glomerular mesangial cells under interleukin -1β. Methods：Preparation BSTLF rat plasma,cell proliferation was determined by MTT,the protein expression of TGF-β1 in glomerular mesangial cells were detected by ELISA,and the mRNA expression of CTGF in glomerular mesangial cells were detected by RT-PCR. Results：Interleu-kin-1βcould induce cell proliferation and enhance expression of TGF-β1 and CTGF in rat glomerular mesangial cells compared with control group（P〈0. 01）. BSTLF could inhibit cell proliferation and expression of TGF-β1 and CTGF in glomerular mesangial cells cultured under interleukin-1β（P〈0. 01）,and time-dependent. Conclusion：BSTLF suppresses interleukin-1β-induced cell proliferation,suppresses TGF-β1 and CTGF expression,regulate function of glomerular mesangial cells to inhibit renal fibrosis.

关 键 词：补肾通络方 肾脏纤维化 肾小球系膜细胞 细胞增殖 TGF-β1 CTGF 

分 类 号：R285.5[医药卫生—中药学]