LOXL2和E-cadherin在胆管癌浸润转移中的表达及相关性  

Expression of E-cadherin and lysyl oxidase-like 2 in cholangiocarcinoma

作　　者：刘鹏[1] 王建华 蒋海滨[3] 彭娅[1] 

机构地区：[1]湖南省人民医院消化内科,湖南省长沙市410005 [2]湖南省常德市第四人民医院消化内科,湖南省常德市415000 [3]南华大学第二附属医院消化内科,湖南省衡阳市421001

出　　处：《世界华人消化杂志》2014年第35期5542-5549,共8页World Chinese Journal of Digestology

基　　金：湖南省科技厅基金资助项目;No.2010SK3068~~

摘　　要：目的:研究赖氨酰氧化酶样蛋白-2(lysy loxidase-like 2 protein,LOXL2)及黏附蛋白E-cadherin在胆管癌的表达,探讨其表达情况与胆管癌生物学行为的关系.方法:应用SP法及RT-PCR技术分别检测47例胆管癌、47例配对的癌旁组织、20例正常胆管组织中LOXL2和E-cadherin的表达情况,对结果应用SPSS17.0统计软件进行.数据进行统计学分析.结果:(1)在47例胆管癌组织、癌旁组织、正常胆管组织中L O X L2蛋白的阳性表达率分别为68.1%、10.6%、10.0%(P<0.05);E-cadherin蛋白阳性表达率分别为21.3%、7 4.5%、9 5.0%,差异均具有统计学意义(P<0.0 5);(2)胆管癌组织、癌旁组织、正常胆管组织中LOXL2 m RNA的表达分别为154.63±128.96、5.35±11.85、4.52±10.79(P<0.05);E-c adherinm RNA的表达分别为0.18±0.25、0.95±0.62、1.08±0.33(P<0.05);(3)胆管癌组织中E-cadherin的表达减弱和LOXL2的表达增强均与患者肿瘤的分化程度、分期、淋巴结转移和组织器官转移相关(P<0.05);(4)胆管癌中E-cadherin和LOXL2的表达呈负相关.结论:在胆管癌组织中E-cadherin的表达低于癌旁组织减弱,而LOXL2表达高于癌旁组织;二者的表达呈负相关且与肿瘤的分化程度有明显相关性.AIM： To examine the expression of lysyl oxidase-like 2 （LOXL2） and E-cadherin in cholangiocarcinoma, and to analyze their relationship with clinicopathological features of cholangiocarcinoma. METHODS： Streptavidin-peroxidase （SP） immunohistochemical method and SYBR green real-time polymerase chain reaction （RT-PCR） were used to detect the protein and mRNA expression of LOXL2 and E-cadherin in 47 cases of cholangiocarcinoma, 47 matched tumor adjacent tissues, and 20 normal bile duct tissues. RESULTS： Expression of LOXL2 protein was significantly higher in cholangiocarcinoma than in matched tumor adjacent tissues and normal bile duct tissues （68.1% vs 10.6%, 10.0%, P 〈 0.05）. Expression of E-cadherin protein was significantly lower in cholangiocarcinoma than in paired tumor adjacent tissues and normal bile duct tissue （21.3% vs 74.5%, 95.0%, P 〈 0.05）. Expression of LOXL2 mRNA was significantly higher in cholangiocarcinoma than in paired tumor adjacent tissues and normal bile duct tissues （154.63 ± 128.96 vs 5.35 ± 11.85, 4.52 ± 10.79, P 〈 0.05）. Expression of E-cadherin mRNA was significantly lower in cholangiocarcinoma than in paired tumor adjacent tissues and normal bile duct tissues （0.18 ± 0.25 vs 0.95 ± 0.62, 1.08 ± 0.33, P 〈 0.05）. Down-regulation of E-cadherin and up-regulation of LOXL2 were related with tumor differentiation, stage, lymph node metastasis and distant organ metastasis （P 〈 0.05）. The expression of E-cadherin was negatively related to that of LOXL2. CONCLUSION： Expression of E-cadherin is significantly lower and that of LOXL2 is significantly higher in cholangiocarcinoma. Expression of E-cadherin and LOXL2 is associated with tumor differentiation, stage, lymph node metastasis and distant organ metastasis in cholangiocarcinoma.

关 键 词：E-CADHERIN 赖氨酰氧化酶样蛋白-2 胆管癌 SP法 RT-PCR 

分 类 号：R735.8[医药卫生—肿瘤]