紫外-可见分光光度法测定火龙果花中总黄酮的含量  

Determination of Total Flavonoids in Pitaya Flower by UV-Vis Spectrophotometry

作　　者：罗小艳[1,2] 郭璇华[1] 

机构地区：[1]华南理工大学化学与化工学院,广东广州510640 [2]广东省绿色化学产品技术重点实验室,广东广州510640

出　　处：《食品研究与开发》2014年第23期108-111,共4页Food Research and Development

摘　　要：研究火龙果花中黄酮类化合物的分离提取和测定方法。在单因素实验的基础上,利用正交实验对分离提取条件进行优化。结果表明,最佳分离提取条件为:乙醇浓度为70%,固液比为1∶40(g/m L),提取温度为75℃,提取时间为2 h;以芦丁为标准品,采用Al Cl3显色-分光光度法测得火龙果花中总黄酮含量为0.747%,样品平均回收率为99.51%,RSD值为2.06%(n=5)。In this paper, the extraction and separation of the total flavones from pitaya flower were studied. Based on single-factor experiment, the extraction process conditions were optimized by using orthogonal test. Results showed that the optimal extraction conditions were as follows：ethanol concentration 70%, ratio of raw material to ethanol solution 1∶40（g/mL）, extraction temperature 75℃and extraction time 2 h. Under the above conditions, the content of total flavones from dry pitaya flower was 0.747%. The average recovery rate and RSD were 99.51%and 2.06%respectively （n=5）.

关 键 词：火龙果花 总黄酮 紫外-可见分光光度法 AlCl3显色法 

分 类 号：S667.9[农业科学—果树学] O657.32[农业科学—园艺学]