柱前衍生化高效液相色谱法测定美沙拉嗪肠溶片中5-ASA的含量  

Determination of 5-Aminosalicylic acid in Mesalazine Enteric-coated Tablets by Precolumn Derivatization of HPLC

作　　者：张晓卿 

机构地区：[1]石药集团维生药业(石家庄)有限公司,河北石家庄050011

出　　处：《煤炭与化工》2014年第11期50-52,共3页Coal and Chemical Industry

摘　　要：采用RP-HPLC测定美沙拉嗪肠溶片中5-氨基水杨酸（5-ASA）的含量。柱前衍生化使样品中5-ASA乙酰化,采用反相高效液相色谱法,色谱柱为ODS C18,流动相为磷酸盐缓冲液-乙腈（92∶8）,流速为0.9 m L·min-1,检测波长为304 nm。结果表明,5-ASA在3.12~50.0μg·m L-1范围内线性关系良好（r=0.999 6）,平均回收率为100.8%,RSD为1.27%,柱前衍生化高效液相色谱法测定美沙拉嗪肠溶片中5-ASA的含量简便、快速、准确、专属性强,适用于美沙拉嗪肠溶片中5-ASA的含量测定。The RP-HPLC method was used to determine the 5-aminosalicylic acid （5-ASA） in mesalazine enteric-coated tablets.5-ASA was derivatized to acetyl 5-ASA, RP-HPLC was adopted with a ODS Cts column, a mobile phase consisting of phosphate buffer-acetonitrile （9 ： 28） at 0.9 mL min-1,and the UV detective wavelength was 304 nm.The calibration curve of 5-ASA was linear in the concentration range of 3.12-50.0μg·m L-1,the average recovery was 100.8% with RSD of 1.27% . The method was credible, and specific the operatin was simple and the result was accurate. The method was applicable to determine the 5-ASA in mesalazine enteric-coated tablets.

关 键 词：美沙拉嗪肠溶片 5-氨基水杨酸(5-ASA) RP-HPLC法 含量 

分 类 号：TQ460.7[化学工程—制药化工]