栀子柏皮汤及其拆方对中医阳黄证黄疸大鼠退黄作用的研究  

Therapeutic Effects of Zhizi Baipi Decoction and Its Separated Prescriptions on Relieving Jaundice of Jaundice Rat with Yang-jaundice syndrome

作　　者：朱继孝[1] 李雪溦 李磊[1,2] 钟国跃[1] 熊浩仲[3] 刘春花[3] 罗光明[1] 

机构地区：[1]江西中医药大学中药资源与民族药研究中心,江西南昌330004 [2]威海诺达药业集团有限公司,山东威海264200 [3]江西中医药大学基础医学院,江西南昌330004

出　　处：《中药新药与临床药理》2015年第1期25-30,共6页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家自然科学基金(81160510)

摘　　要：目的研究栀子柏皮汤及其拆方对中医阳黄证模型大鼠的治疗作用及机制,并探讨组方配伍规律,为临床使用该方提供实验依据。方法采用以异硫氰酸萘酯(ANIT)灌胃诱导大鼠肝损伤基础上,结合高温高湿的环境因素、高糖高脂的饮食,复制阳黄证大鼠模型,灌胃给予栀子柏皮汤及其拆方,观察给药后大鼠的一般生物学状态,检测血清中丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、总胆红素(TBIL)、胆汁酸(TBA)和补体C3、C4水平,以及肝组织中总超氧化物歧化酶(T-SOD)活性和脂质过氧化产物丙二醛(MDA)含量,光镜下观察大鼠肝组织病理变化。结果与正常组比较,阳黄证模型组各指标均有明显变化;与阳黄证模型组比较,阳性药茵栀黄颗粒组及栀子柏皮汤组、栀子黄柏组大鼠血清中ALT、AST、TBIL、TBA和补体C3、C4水平均显著降低(P<0.05,P<0.01);栀子甘草组、栀子组大鼠血清中ALT、AST、TBA的活性和补体C3、C4水平均显著降低(P<0.05,P<0.01);仅栀子甘草组大鼠肝脏MDA含量显著降低(P<0.05);黄柏甘草给药组能显著降低大鼠血清中补体C3、C4表达水平(P<0.05,P<0.01);各给药组均能显著提高肝脏SOD活性(P<0.05,P<0.01)。各拆方组大鼠血清中ALT、AST、TBIL、TBA含量均高于栀子柏皮汤,其中缺栀子的黄柏甘草组、黄柏组以及甘草组与栀子柏皮汤组比较差异有统计学意义(P<0.05)。病理切片显示栀子柏皮汤及其拆方组可不同程度的缓解肝损伤。结论栀子柏皮汤对阳黄证模型大鼠具有明显的治疗作用,其退黄机制可能为抑制炎症反应、清除自由基,从而减轻肝脏损伤;拆方结果显示全方疗效优于其他拆方组,初步阐明了栀子柏皮汤组方配伍的科学性、合理性。Objective To investigate the effects of Zhizi Baipi Decoction,mainly composed of Zhizi（Fructus Gardeniae）,Gancao（Radix Glycyrrhizae）,Y inchen（Herba Artemisiae Scopariae）and Huangbai（Cortex Phellodendri）, and its separated prescriptions on rat model with yang-jaundice syndrome and to explore the mechanism of prescription compatibility. Methods Rat model with yang-jaundice syndrome was induced by α-naphthyl isothiocyanate（ANIT） intragastrically, along with the hot and humid environment and high-fat and high-carbohydrate diet.Zhizi Baipi Decoction and its separated prescriptions in different dosages were administrated intragastrically to the model rats. After treatment, the serum levels of alanine aminotransferase（ALT）, aspartate aminotransferase（AST）, total bilirubin （TBIL）,bile acid（TBA）,complement C3 and C4 as well as hepatic total superoxide dismutase（T-SOD）activity and malonaldehyde（MDA） content were detected. Meanwhile, the hepatic pathological changes were observed under the light microscope. Results Compared with the normal control group,the differences of the observation indexes in model group were significant statistically（P〈0.05, P〈0.01）. Compared with model group, positive drug（Y inzhihuang Granule）group,Zhizi Baipi Decoction group and Zhizi-Huangbai group had obviously low serum ALT,AST,TBIL, TBA,complement C3,C4 levels （P〈0.05,P〈0.01）, Zhizi-Gancao group and Zhizi group had low serum ALT, AST,TBA,complement C3,C4 levels （P〈0.05,P〈0.01）,Zhizi-Gancao group had low hepatic MDA content （P〈0.05）,and Huangbai-Gancao group had low serum complement C3,C4 levels（P〈0.05,P〈0.01）. SOD activity was improved in all of the medication groups（P〈0.05,P〈0.01）. Compared with Zhizi Baipi Decoction group,the separated prescriptions groups had high ALT, AST, TBIL and TBA levels in blood serum, the differences in Huangbai-Gancao group vs Huangbai group,Gancao group vs Zhizi Baipi Decoction being significant（P〈0.05）. The result

关 键 词：栀子柏皮汤 拆方 阳黄证 生化指标 病理学 

分 类 号：R285.5[医药卫生—中药学]