23-乙酰泽泻醇B对SGC-7901细胞线粒体能量代谢的影响  

Effect of Alisol B 23-acetate on SGC-7901 Cell Mitochondrial Energy Metabolism

作　　者：林文津 徐榕青 张亚敏 李主泉 宋晓瑞 

机构地区：[1]福建省医学科学研究院福建省医学测试重点实验室,福建福州350001

出　　处：《中药新药与临床药理》2015年第1期30-34,共5页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家自然科学基金(81102875);福建省省属公益类科研院所基本科研专项(2013R0034-3;2014R1031-7);福建省卫生系统中青年骨干人才培养项目(2014-ZQN-JC-9)

摘　　要：目的观察中药泽泻中指标成分23-乙酰泽泻醇B对细胞线粒体能量代谢的影响。方法以SGC-7901胃癌细胞为研究对象,绘制其生长曲线,MTT法检测细胞增殖抑制率,以阳离子荧光羰花青染料JC-1染色,采用倒置荧光显微镜观察结合流式细胞仪检测对细胞线粒体膜电位;多功能酶标仪检测琥珀酸脱氢酶（SDH）活性。结果 23-乙酰泽泻醇B 3.125μg·m L-1干预能促进细胞增殖,23-乙酰泽泻醇B 25~100μg·m L-1干预则不同程度的抑制细胞增殖,并且呈浓度依赖关系。与正常对照组比较,不同剂量组的红色荧光和绿色荧光的变化不明显。流式细胞仪检测结果显示,正常对照组、23-乙酰泽泻醇B低、中、高剂量组的单阳细胞比例分别为3.5%,3.6%,3.7%和4.0%,与正常对照组比较,23-乙酰泽泻醇B低剂量组单阳细胞比例增加,但差异无统计学意义（P〉0.05）,23-乙酰泽泻醇B中、高剂量组单阳细胞比例增加,差异有统计学意义（P〈0.05,P〈0.01）;SDH检测结果显示,正常对照组、23-乙酰泽泻醇B低、中、高剂量组的SDH活性分别为0.045、0.058、0.080、0.100μmol·min-1,与正常对照组比较,23-乙酰泽泻醇B低剂量组的SDH活性差异无统计学意义（P〉0.05）,23-乙酰泽泻醇B中、高剂量组的SDH活性差异有统计学意义（P〈0.05,P〈0.01）。结论 23-乙酰泽泻醇B在低剂量时能促进SGC-7901细胞增殖,中剂量与高剂量则对SGC-7901线粒体膜电位有一定的损伤而抑制增殖,但对SDH活性促进作用较显著,提示其具有较明显促进线粒体能量代谢的作用,为进一步探讨泽泻改善线粒体能量代谢物质基础的研究奠定了基础。Objective To observe the effect of alisol B 23-acetate（ABA）, an effective component of Rhizoma Alismae,on mitochondrial energy metabolism of SGC-7901 cells. Methods Human gastric carcinoma SGC-7901 cells were exposed to alisol B 23-acetate at different concentrations. The inhibitive rate of cell proliferation was measured by MTT assay and the cell growth curve was set up, SGC-7901 cells were dyed by cation fluorescence carbocyanine JC-1, the mitochondrial membrane potential was determined by the inverted fluorescence microscope and flow cytometer, and the succinate dehydrogenase（SDH） activity was detected by microplate reader. Results Alisol B 23-acetate at 3.125 μg/mL promoted cell proliferation, while alisol B 23-acetate at 25-100 μg/mL inhibited the proliferation of SGC-7901 cell line in a dose-dependent manner. Compared with the normal control group（NCG）,the changes of red fluorescence weakening（or green fluorescence enhancing） in alisol B 23-acetate groups were not obvious. The proportion of single positive cells was 3.5 %, 3.6 %, 3.7 % and 4.0 % in NCG, and low-dose, medium-dose, and high-dose ABA groups, respectively, and the differences of NCG with medium- and high-dose ABA groups were significant（P 〈 0.05, P 〈 0.01）. SDH activities of NCG, and low-dose, medium-dose, and high-dose ABA groups were 0.045,0.058,0.080,0.100 μmol＆#183;min-1,respectively,and the differences of NCG with medium- and high-dose ABA groups were significant（P 〈 0.05, P 〈 0.01）. Conclusion Low dose of alisol B 23-acetate would promote SGC-7901 cell proliferation, while medium dose and high dose of alisol B 23-acetate would inhibit SGC-7901cells proliferation and accelerate SGC-7901cells SDH activity. It is implied that alisol B 23-acetate could improve the mitochondrial energy metabolism, which lay the foundation for investigating the therapeutic mechanism of Rhizoma Alismae on improving mitochondrial energy metabolism.

关 键 词：23-乙酰泽泻醇B 线粒体 能量代谢 膜电位 琥珀酸脱氢酶 

分 类 号：R285.5[医药卫生—中药学]