对二甲基亚砜在小鼠淋巴瘤细胞实验中适宜浓度的探索  

An optimal concentration range of dimethyl sulfoxide used in mouse lymphoma assay

作　　者：胡燕平[1] 文海若 宋捷[1] 左泽平[2] 许雷鸣[3] 

机构地区：[1]中国食品药品检定研究院国家药物安全评价监测中心,北京100176 [2]北京市药品检验所,北京100035 [3]安徽省食品药品检验所,合肥230051

出　　处：《中国新药杂志》2015年第2期208-211,共4页Chinese Journal of New Drugs

摘　　要：目的:通过采用小鼠淋巴瘤细胞实验微孔法,检测二甲基亚砜(DMSO)的细胞毒性及遗传毒性,探索其在检测系统中的适宜浓度。方法:在非代谢活化(-S9)条件下,检测美国Sigma和Tedi A两家公司生产的2个批号DMSO,终浓度0.5%,1.0%,2.0%,4.0%处理L5178Y细胞3 h后产生的细胞毒性及诱发的TK基因突变频率(MF)。结果:2个批号DMSO的细胞毒性均以1%浓度组为最低(<20%),4%浓度组为最高(>37%);1%浓度组诱发的MF与空白对照组相近,而其他3组则明显增加(P<0.05或0.01)。结论:当以DMSO为溶媒、采用L5178Y细胞进行MLA微孔法实验时,采用1%作为检测系统中受试物的体积比浓度较为适宜。Objective： To investigate the cytotoxicity and genotoxicity of dimethyl sulfoxide（ DMSO）,and an applicable range of concentration used in mouse lymphoma assay（ MLA）. Methods： DMSO purchased from Tedia（ DMSO-I） and Sigma（ DMSO-II）,respectively,were compared at final concentrations of 0. 5%,1. 0%,2. 0% and 4. 0% without the metabolic activation of S9. The cytotoxicity and mutation frequency（ MF） of tk gene induced by both DMSO subjected to a 3 h treatment in L5178 tk＋ /-cells were statistically analyzed. Results： For both batches of DMSO,1% showed the lowest cytotoxicity（〈 20%）,while 4% exhibited the highest（ 〉37%）.MF triggered by 1% DMSO was similar to that of the blank control,however MF ratios were significantly increased by other concentrations（ P〈0. 05 or 0. 01）. Conclusion： 1% is the optimal concentration for DMSO as a solvent applied in the L5178 Y tk^＋/- cell line and micro-well plates based MLA test.

关 键 词：二甲基亚砜 小鼠淋巴瘤细胞实验 细胞毒性 遗传毒性 适宜浓度 

分 类 号：R994[医药卫生—毒理学]