GC-MS结合保留指数分析桑白皮脂肪油成分  

Analysis of fatty oil in cortex mori by GC-MS in combination with kovats retention index

作　　者：郑晓珂[1] 王绅[1] 张明辉[1] 王小兰[1] 赵威[1] 冯卫生[1] 

机构地区：[1]河南中医学院,郑州450046

出　　处：《中国新药杂志》2015年第2期228-230,234,共4页Chinese Journal of New Drugs

基　　金：国家重点基础研究发展计划(973计划)(2013CB531802)

摘　　要：目的:运用GC-MS结合保留指数分析桑白皮脂肪油成分,为进一步研究桑白皮脂肪油提供实验依据。方法:以石油醚为溶剂,采用索氏提取法提取脂肪油,经甲酯化处理后,用GC-MS技术并结合保留指数对其进行分析鉴定。结果:采用GC-MS分析,检测出38个组分,利用MS结合保留指数定性,鉴定出其中27个成分。含量较高的组分为:角鲨烷(50.40%)、α-香树脂素(10.86%)、亚油酸甲酯(9.33%)、β-香树脂素(4.84%)、棕榈酸甲酯(3.08%)、11-十八烯酸甲酯(1.76%)等。结论:本实验方法可靠,结果准确度高,为开发利用桑白皮资源提供依据。Objective： To analyze the fatty oil in cortex mori for providing an experimental basis for further research on activity of mulberry fatty oil. Methods： Petroleum ether was used as solvent to extract fatty acids by Soxhlet extraction method. After methyl esterification,the analysis and identification were made by gas chromatography-mass spectrometry（ GC-MS） technology in combination with retention indices. Results： Thirty-eight components were detected by GC-MS and 27 compounds were identified by MS in combination with kovats retention index. The compounds with high contents were as follows： squalane（ 50. 40%）,α-Amyrin（ 10. 86%）,methyl linoleate（ 9. 33%）,β-Amyrin（ 4. 84%）,hexadecanoic acid methyl ester（ 3. 08%）,11-Octadecynoic acid methyl ester（ 1. 76%） and so on. Conclusion： The experimental method is reliable,accurate and provides the basis for the development and utilization of morus alba resources.

关 键 词：桑白皮 脂肪油 GC-MS 保留指数 

分 类 号：R284.1[医药卫生—中药学]