丁苯酞预处理对低压低氧小鼠记忆、海马NO和ET-1含量的影响  

Effects of butylphthalide on NO and ET-1 in brain tissues and memory in hypobaric hypoxia mice model

作　　者：刘静[1] 宋永斌[1] 杨俊[1] 郑俊[1] 马孝俊[1] 杨遥[1] 钟玲[1] 徐江涛[1] 

机构地区：[1]兰州军区乌鲁木齐总医院神经内科,新疆乌鲁木齐830000

出　　处：《西北国防医学杂志》2014年第3期211-214,共4页Medical Journal of National Defending Forces in Northwest China

基　　金：全军"十一五"计划课题项目(06MB106)

摘　　要：目的:观察丁苯酞(NBP)预处理对急性低压低氧小鼠空间记忆功能、脑组织一氧化氮(NO)和内皮素-1(ET-1)含量的影响。方法:42只成年昆明小鼠被随机分为3组,即常压常氧对照组、低压低氧对照组和低压低氧实验组,通过Morris水迷宫实验的逃跑潜伏时间和平均速度评估小鼠空间记忆功能,并检测海马组织中的NO和ET-1含量。结果:与常压常氧组比较,低压低氧对照组和低压低氧实验组的潜伏期明显延长,平均速度显著变慢,低压低氧对照组脑组织NO和ET-1含量均显著增加,低压低氧实验组脑组织ET-1含量显著增加(P<0.05或<0.01);与低压低氧对照组比较,低压低氧实验组的潜伏期显著缩短,平均速度显著增快,脑组织ET-1含量显著减少(P<0.05)。结论:①低压低氧可致小鼠空间记忆功能受损,这可能与低压低氧所致的小鼠脑组织NO和ET-1异常有关;②丁苯酞预处理可以减轻低压低氧对小鼠记忆功能的损害,可能与丁苯酞减轻低压低氧所致的小鼠脑组织ET-1异常有关。Objective： To observe the effects,; of butylphthalide （dl--3 --n- butylphthalide, NBP） preconditioning on spatial memory and the levels of nitric oxide （NO） and endothelin （ET--1） of the brain tissue in mice exposed to acute hypobaric--hypoxia. Methods： Forty--two Kunming mice were divided randomly into 3 groups, normobaric normoxia control group, hypobaric hypoxia control group and hypo- baric hypoxia experimental group. Escape latence and average velocity of Morris water maze test were em- ployed to evaluate spatial memory and the content of NO and ET--1 in the brain. Results： Compared to normobaric normoxic control group, a significant increase in escape latence and decrease in average velocity were found in hypobaric hypoxia control and experimental group （P〈0.05 or P〈0.01）. Compared to normobaric normoxic control group, a significant increase in content of ET--1 and NO in the brain were found in hypobaric hypoxia control group and a significant increase in content of ET-- 1 were found in hy- pobaric hypoxia experimental group（ P〈0.05 or P〈0.01）. Compared to hypobaric hypoxia control group, a significant decrease in escape latence and a significant increase in average velocity and content of ET--1 in the brain were found in hypobaric hypoxia experimental group （P〈0. 05）. Conclusion： The spatial memo- ry could be impaired by exposing to acute hypobaric--hypoxia, which may be related to the elevation of the levels of NO and ET-- 1 in brain tissue. Butylphthalide preconditioning can partially reverse of memory im- pairment caused by exposing to acute hypobaric--hypoxia, which may be related to the depression of the levels of ET--1 in brain tissue.

关 键 词：高原 丁苯酞 低压低氧 记忆功能 一氧化氮 内皮素 

分 类 号：R594.3[医药卫生—内科学]