戊巴比妥钠在2型糖尿病大鼠模型复制中的应用研究  

Application research of pentobarbital sodium in rats model of type 2 diabetes mellitus

作　　者：刘华[1] 刘行海[1] 冯乾[1] 张婷[1] 买文丽[1] 兰海涛[1] 郑倩[1] 刘红[1] 

机构地区：[1]川北医学院生理学教研室,四川南充637100

出　　处：《川北医学院学报》2014年第5期425-428,共4页Journal of North Sichuan Medical College

基　　金：四川省教育厅重点项目(2010ZA169);四川省教育厅项目(2011ZB159);川北医学院创新团队项目(CBY13-7D02)

摘　　要：目的:研究戊巴比妥钠在2型糖尿病大鼠模型复制中的应用效果。方法:雄性Wistar大鼠,实验组采用"高脂饲养+戊巴比妥钠+链脲佐菌素(streptozotocin,STZ)"的方法复制2型糖尿病模型,对照组采用"高脂饲养+STZ"的方法复制模型,各时期以空腹血糖>7.8mmol·L-1作为2型糖尿病模型的判断标准。结果:(1)实验组大鼠胰腺与肝脏组织呈现典型的糖尿病病理改变;(2)STZ注射后第2、4、6、8周比较,实验组大鼠成模率都显著高于对照组(P<0.01);(3)在STZ注射后第6和8周,实验组死亡率显著低于对照组(P<0.01);(4)实验各期血糖变异度比较,实验组大鼠显著低于对照组(P<0.01)。结论:戊巴比妥钠结合小剂量STZ可以提高2型糖尿病模型的成功率和稳定性,降低死亡率和模型之间的血糖差异性。Objective：To study the application effects of pentobarbital sodium in rats model of type 2 diabetes mellitus（T2DM）. Meth-ods：Male Wistar rats were used in the experiment. The experimental group rats were used in the“high-fat chow ＋ pentobarbital sodium＋ streptozotocin（ STZ）”to copy the model of type 2 diabetes. The control group rats were used in the“high-fat chow ＋ STZ”to copy the model. Rats with fasting blood glucose〉7. 8 mmol·L-1 were taken as standard of T2DM. Results：（1）The experimental group rats’ pan-creas and livers tissue showed changes in diabetic pathological type;（2）After injecting STZ,compared the 2nd,4th,6th and 8th week,the success rate of the experimental group is significantly higher than that of the control group（P〈0. 01）;（3）The 6th and 8th week,the death rate of the experimental group is significantly lower than the control group（P〈0. 01）;（4）The glucose variability of the experimental group is significantly lower than those in the control group（P〈0. 01）. Conclusion：Pentobarbital sodium combined with low doses of STZ can improve the success rate and stability of model of type 2 diabetes and reduce the mortality and glucose variability.

关 键 词：戊巴比妥钠 2型糖尿病 模型 应用效果 大鼠 

分 类 号：R331[医药卫生—人体生理学] R587[医药卫生—基础医学]