南充市钩体患者血清IFN-γ、TNF-α、IL-10动态观察  

The dynamic of TNF-α,IL-10,IFN-γ in serum of patients with leptospirosis

作　　者：张廷君[1] 周仲辉[1] 曾跃[1] 刘凤君[1] 王贵霞[1] 林家福[1] 

机构地区：[1]川北医学院附属医院感染科,四川南充637000

出　　处：《川北医学院学报》2014年第5期458-461,共4页Journal of North Sichuan Medical College

基　　金：四川省卫生厅科研项目(100150)

摘　　要：目的:了解钩体病人病程不同时期血清中IFN-γ、IL-10、TNF-α动态变化特征。方法:通过MAT、钩体分离培养、PCR检查确定实验室诊断病例,再通过酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)检测血清IFN-γ、TNF-α、IL-10的水平。结果:实验室确诊病例24例。早、中、晚期IFN-γ水平均较正常人高(P<0.05),早、中、晚期IFN-γ水平无差别;早、中期TNF-α水平较正常人高(P<0.05),晚期TNF-α水平与正常人无差别(P>0.05),中期较早、晚期高(P<0.05),早期与晚期无差别(P>0.05);早、中、晚期IL-10水平均较正常人高(P<0.05),早期较中期高,中期较晚期高(P<0.05)。结论:三种细胞因子IFN-γ、TNF-α、IL-10水平在钩体病的病程中呈现出各自的变化规律,提示IFN-γ、IL-10和TNF-α可能在机体清除钩体及疾病的发展过程中均起着重要的作用。Objective：To investigate the dynamic of serum TNF-α,IL-10,IFN-γ of patients with leptospirosis. Methods：The labora-tory-confirmed cases were defined by microscopic agglutination test（MAT）,fractional cultivation and polymerase chain reaction,then the levels of TNF-α,IL-10,IFN-γwere detected by enzyme-linked immunosorbent assay（ELISA）. Results：There were 24 samples con-firmed. The levels of IFN-γof patients in early,middle,late period were all higher than that of control（P〈0. 05）. Significant statistics difference was not found in multiple comparison of IFN-γ among early,middle and late cases（P〉0. 05）. The level of TNF-α in early and middle period was higher than that of control group（P〈0. 05）,the difference of the level TNF-α between late period and normal human was not statistically significant（P〉0. 05）,The paired comparison confirmed that TNF-α levels of medium-term is higher than that of early and late period（P〈0. 05）,and the difference between early and late was not statistically significant（P〉0. 05）. The levels of IL-10 early,middle,late period were all higher than normal（P〈0. 05）. The level of IL-10 in late period was lower than that of mid-dle period,and the level of IL-10 in middle period was higher than that of early period（P〈0. 05）. Conclusion：The level of IFN-γ, TNF-αand IL-10 expressed their one rules in the whole leptospirosis course,respectively. It is suggested that IFN-γ,TNF-α,and IL-10 play important roles in the course of leptospira clearance and process of leptospirosis.

关 键 词：钩端螺旋体病 IFN-Γ TNF-Α IL-10 

分 类 号：R514.4[医药卫生—内科学]