人乳头瘤病毒基因分型检测阳性率与其核酸载量的相关性研究  

Correlation analysis of positive rate of HPV genotyping test and HPV nucleic acid loads

作　　者：汪东剑[1] 张晓云[1] 刘冬萍[1] 冯丽霞[1] 邱华琴[1] 徐波[1] 

机构地区：[1]中国人民解放军第一八四医院输血科,鹰潭335000

出　　处：《中华微生物学和免疫学杂志》2014年第12期950-953,共4页Chinese Journal of Microbiology and Immunology

摘　　要：目的：探讨PCR-反向点杂交基因分型检测阳性率与病毒核酸载量间的关系。方法采用PCR荧光法对1162例女性患者进行HR-HPV DNA载量检测，采用PCR-反向点杂交法对其中141例高危HR-HPV DNA阳性标本进行HPV基因分型检测。结果基因分型检测总阳性率为68.8％，基因分型检测阳性率与HR-HPV DNA载量对数值呈显著正相关（r＝0.944，P﹤0.01），其二次项曲线拟合公式为Y＝-1.806＋0.558X-0.031X2（Y为基因分型阳性率，X为HR-HPV DNA载量对数值）。不同载量组间的基因分型检测阳性率差异具有统计学意义（P﹤0.01）：当HR-HPV DNA载量在10^4～10^5拷贝/ml、10^5～10^6拷贝/ml、10^6～10^7拷贝/ml和＞10^7拷贝/ml时，使用不同厂家基因分型检测试剂其阳性率分别为27.8％、48.5％、74.0％、97.5％和33.3％、51.5％、78.0％、97.5％。结论PCR-反向点杂交法基因分型检测阳性率与其核酸载量相关。Objective To investigate the correlation between the positive rate of PCR-reverse dot blot genotyping test and the loads of the viral nucleic acid. Methods The fluorescent PCR assay was used to detect the high-risk HPV（HR-HPV）DNA loads in the cervical mucus samples from 1162 female patients. Patients with positive HR-HPV DNA were further analyzed by PCR-reverse dot blot hybridization assay for HPV genotyping. Results The overall positive rate of genotyping test was 68. 8% . The positive rate of genotyping test had a significant positive correlation with the Log Koc of HR-HPV DNA loads（r=0. 944, P﹤0. 01）. The quadratic curve fitting formula was Y= -1. 806＋0. 558X-0. 031X2（Y for genotyping positive rate,X for Log Koc of HR-HPV DNA loads）. There were significant differences with the positive rate of genotyping test among patients with different viral loads（P﹤0. 01）. When HR-HPV DNA loads were 10^4-10^5 copies/ ml,10^5-10^6 copies/ ml,10^6-10^7 copies/ ml and more than 10^7 copies/ ml,the positive rate of HPV genotyping test were 27. 8% ,48. 5% ,74. 0% ,97. 5% and 33. 3% ,51. 5% ,78. 0% ,97. 5% respectively by using different genotyping detection reagents. Conclusion The positive rate of PCR-reverse dot blot genotyping test was correlated with the loads of HPV nucleic acid.

关 键 词：人乳头瘤病毒 病毒载量 聚合酶链反应 基因分型 反向点杂交 

分 类 号：R440[医药卫生—诊断学]