mTOR信号通路在人子宫肌和子宫肌瘤组织中的活性研究  

The activity of m TOR signal pathway in human myometrium and uterine leiomyoma

作　　者：周玉[1] 许丽娟[2] 王天齐[2] 马迪[2] 李阳[2] 李宗金[2] 王悦冰[2] 

机构地区：[1]安徽省蚌埠医学院第一附属医院妇产科,蚌埠233000 [2]南开大学医学院,天津300071

出　　处：《现代妇产科进展》2014年第12期942-945,共4页Progress in Obstetrics and Gynecology

基　　金：南开大学本科生创新科研计划项目(No:ZB140047);南开大学基础科研业务费(No:65011281)

摘　　要：目的:检测哺乳动物雷帕霉素靶分子(m TOR)信号通路中相关蛋白在人子宫肌和子宫肌瘤组织中的表达。方法:收集22例子宫肌和与之配对的子宫肌瘤组织,采用Western blot法和Real-time PCR法检测tuberin、hamartin、p-S6K、p-4EBP1和p-Akt表达。结果:与子宫肌比较,子宫肌瘤中tuberin、S6K和4EBP1呈高表达,hamartin表达不升高,p-S6K和p-4EBP1表达降低,Akt和p-Akt表达无显著变化。结论:Tuberin在子宫肌瘤中高表达;m TOR信号通路在子宫肌瘤中受到抑制。Objective：To investigate the activation of mTOR （mammalian target of rapamycin） signal pathway related proteins in human myometrium and their matched uterine leio- myomas. Methods： The expression levels of tuberin, hamartin, p-S6K ,p-4EBP1 and p-Akt were detected＇in human uterine leiomyoma and their matched myometfial tissues frbm 22 Chi＇nese patients by Western blot and Real-time polymerase chain reaction （RT-qPCR） analyses. Results： The expression of tuberin, but not hamartin, was significantly increased in leiomyoma tissues compared with the matched normal myometrium. The phosphorylation levels of both S6K and 4EBP1 were down-regulated in human leiomyoma. However, the mRNA levels of S6K and 4EBP1 were up-regulated in human leiomyoma. There were no statistical differences between the expression levels of Akt and p-Akt in both tissues. Conclusion：The expression of tuberin was significantly increased in leiomyoma tissues with the inhibition of the mTOR signaling pathway.

关 键 词：子宫肌瘤 TUBERIN 哺乳动物雷帕霉素靶分子(m TOR) 

分 类 号：R711.74[医药卫生—妇产科学]