褪黑素对慢性间歇性缺氧性心肌cTnT及Ca^(2+)-ATPase的影响  

Effect of melatonin on the cTnT and Ca^(2+)-ATPase of chronic intermittent hypoxia cardiac muscular tissues

作　　者：董译元[1] 凌月福[1] 张耀庭[1] 

机构地区：[1]桂林医学院附属医院耳鼻咽喉头颈外科,广西桂林541001

出　　处：《山东大学耳鼻喉眼学报》2014年第5期1-3,6,共4页Journal of Otolaryngology and Ophthalmology of Shandong University

基　　金：广西壮族自治区卫生厅科研课题(Z2010285)

摘　　要：目的探讨褪黑素(MLT)对常压下慢性间歇性缺氧SD大鼠心肌的保护作用。方法将24只雄性SD大鼠(5周龄)随机分成3组:对照组、实验A组、实验B组。对照组在空气中正常饲养,实验A组和实验B组在常压慢性间歇性低氧箱内,对照组和实验B组灌服生理盐水,实验A组灌服褪黑素。8周后用光电比色法测定血清肌钙蛋白T(cTnT)的浓度和心肌钙离子ATP酶(Ca2+-ATPase)的活性,取大鼠右心室做病理切片。结果 1实验B组与实验A组比较:实验B组的cTnT明显增加(P<0.05),心肌组织的Ca2+-ATPase的活性明显降低(P<0.05)。病理切片显示,个别心肌细胞核消失,心肌细胞肥大,心肌之间有大量出血;2实验A组与对照组比较:cTnT和Ca2+-ATPase均无明显差异(P>0.05)。病理切片显示,心肌细胞之间有少量出血;3实验B组与对照组比较:实验B组的cTnT明显增加(P<0.05),心肌组织的Ca2+-ATPase的活性明显降低(P<0.05)。病理切片显示,个别心肌细胞核消失,心肌细胞肥大,心肌之间有大量出血。结论褪黑素对慢性间歇性缺氧心肌具有保护作用。Objective To investigate whether the melatonin （melatonin,MLT）could protect SD rats’cardiac muscular tissues from chronic intermittent hypoxia damage in the atmospheric pressure.Methods The 24 male,SD rats（5 weeks）were randomly divided into three groups：the Control group,the experimental group A and the experimental group B.The control group rats were fed with normally reared in the air,and the experimental group A and B rats were living in the atmospheric pressure and chronic intermittent hypoxia boxes.The control group rats and the experimental group B rats were fed with normal saline solution （NS ）,and the experimental group A rats were fed with melatonin （MLT）.After 8 weeks,the cTnT capacity and Ca^2＋-ATPase activity of each rat were detected,and the right ventricles were taken for pathological section.Results ①Comparison of the experimental group B and the experimental group A：The cTnT capacity of the experimental group B was increased （P〈0.05）,and the Ca^2＋-ATPase activity of the experimental group B was decreased （P〈0.05 ）.Individual myocardial cell nuclei disappeared.Myocardial cells were hypertrophy and there was a large amount of bleeding between myocardial.②Comparison of the experimental group A and the control group：The above indicators had no obvious differences （P〉0.05）.There was a small amount of bleeding between myocardial.③Comparison of the experimental group B and the control group：The cTnT capacity of the experimental group B was increased （P〈0.05）,and the Ca^2＋-ATPase activity of the experimental group B was decreased （P〈0.05）.Individual myocardial cell nuclei disappeared.Myocardial cells were hypertrophy,and there was a large amount of bleeding in myocardial.Conclusion MLT can protect rats’cardiac muscular tissue from injury of chronic intermittent hypoxia.

关 键 词：褪黑素 慢性间歇性缺氧 肌钙蛋白T 心肌钙离子ATP酶 

分 类 号：R762[医药卫生—耳鼻咽喉科]