薯蓣皂苷元对THP-1巨噬细胞ABCA1表达及胆固醇流出的影响  

Effect of Diosgenin on ABCA1 Expression and Cholesterol Efflux in THP-1-derived Macrophage

作　　者：吕运成[1,2] 王佐[1] 姚峰[1] 谢巍[1,2] 唐艳艳[1] 欧阳宇甲 唐朝克[1] 

机构地区：[1]南华大学医学院心血管疾病研究所 动脉硬化学湖南省重点实验室 生命科学研究中心,湖南省衡阳市421001 [2]南华大学医学院临床应用解剖研究室,湖南省衡阳市421001

出　　处：《中国动脉硬化杂志》2014年第7期680-684,共5页Chinese Journal of Arteriosclerosis

基　　金：湖南省自然科学基金资助项目(14JJ2091);湖南省教育厅资助科研项目(12C0339)

摘　　要：目的探讨薯蓣皂苷元(Dgn)对巨噬细胞ATP结合盒转运体A1(ABCA1)表达及其介导胆固醇流出的影响。方法用Dgn或结合ABCA1小干扰RNA(siRNA)处理THP-1源性巨噬细胞24 h后,Western blot检测ABCA1蛋白水平,HPLC检测胞内总胆固醇(TC)、游离胆固醇(FC)及胆固醇酯(CE)含量,液体闪烁计数仪检测胞内胆固醇流出,油红O染色观察胞内脂滴情况。结果 Dgn呈浓度依赖性上调巨噬细胞ABCA1的表达,促进胞内胆固醇流出,降低胞内TC、FC和CE含量,抑制胞内脂质蓄积和泡沫细胞形成。而ABCA1 siRNA与Dgn共处理细胞后,明显抑制Dgn促胆固醇流出作用,胞内脂质蓄积和泡沫细胞形成明显加剧。结论 Dgn通过促进巨噬细胞ABCA1表达和胆固醇流出,抑制胞内脂质蓄积和泡沫细胞形成。Aim To investigate the effect of diosgenin（Dgn） on ABCA1 expression and cholesterol efflux in THP-1 derived macrophage. Methods ABCA1 expression was detected by Western blot. Cholesterol efflux was detected with liquid scintillator; intracellular total cholesterol（TC）,free cholesterol（FC） and cholesterol ester（CE） were determined with HPLC; and intracellular lipid droplet was stained with oil red O in THP-1-derived macrophage treated with Dgn,alone or in combination with ABCA1 siRNA. Results Dgn upregulated macrophage ABCA1 expression in dosedependent manner. Dgn obviously facilitated macrophage cholesterol efflux,resulting in a decrease in intracellular level of TC,FC,CE and lipid droplet,and the foam cell formation. However,the action of Dgn-facilitating cholesterol efflux was almost entirely abrogated in THP-1 derived macrophage coincubated with ABCA1 siRNA,causing excessive lipid accumulation and increase formation of foam cells. Conclusion Dgn promotes macrophage ABCA1 expression and cholesterol efflux,which suppresses intracellular lipid accumulation and foam cell formation.

关 键 词：薯蓣皂苷元 ATP结合盒转运体A1 巨噬细胞 胆固醇流出 

分 类 号：R96[医药卫生—药理学]