CD147基因型及血浆水平与ST段抬高型心肌梗死的相关性  

The Plasma CD147 Level and Genotypes in Patients with ST-segment Elevation Myocardial Infarction

作　　者：毛郁[1] 严金川[1] 王翠平[1] 王中群[1] 刘培晶[1] 袁伟[1] 

机构地区：[1]江苏大学附属医院心内科,江苏省镇江市212001

出　　处：《中国动脉硬化杂志》2014年第7期695-700,共6页Chinese Journal of Arteriosclerosis

基　　金：国家自然科学基金项目(81170279;81370409);江苏省科教兴卫工程项目(LJ201116);镇江市心血管病重点实验室项目(SS2012002)

摘　　要：目的探讨ST段抬高型心肌梗死(STEMI)患者CD147基因3'非翻译区(UTR)rs8259位点多态性,并观察CD147的基因型及其血浆水平与STEMI的相关性。方法以162例STEMI患者和328例正常对照者作为研究对象,应用聚合酶链反应-限制性片长多态性(PCR-RFLP)技术和DNA测序法检测CD147基因rs8259位点的基因型,酶联免疫吸附实验(ELISA)检测血浆CD147水平。结果 STEMI组血浆CD147水平(4.18±0.95 pg/L)显著高于对照组(2.55±0.29 pg/L)(P<0.01)。CD147基因rs8259位点存在3种基因型(AA、AT、TT),基因型和等位基因频率在STEMI组和对照组分布差异有显著性(P<0.05)。对多个危险因素行Logistic回归分析显示AT基因型(OR=0.346,95%CI:0.210～0.569,P<0.05)和TT基因型(OR=0.107,95%CI:0.046～0.251,P<0.05)能降低STEMI发病易感性的风险。T等位基因携带者患STEMI的相对风险度明显降低(OR=0.543,95%CI:0.404～0.730,P<0.05)。A等位基因携带者患STEMI的相对风险度升高(OR=1.841,95%CI:1.370～2.464,P<0.05),携带T等位基因的STEMI患者血浆CD147水平相比于不携带者明显降低(P<0.05)。结论 CD147基因3'UTR rs8259 A等位基因可能是STEMI发病的易感基因;AA基因型促进CD147高表达进而增加STEMI发病风险,携带T等位基因通过降低CD147表达而降低STEMI的发病风险。Aim To analyze the polymorphism of the CD147 gene 3’UTR rs8259 in patients with ST-segment elevation myocardial infarction（STEMI）,and to study the relation between plasma levels,genotype of CD147 and STEMI.Methods The polymorphism of CD147 was detected by polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） and DNA sequencing method in 162 STEMI patients and 328 healthy persons. The plasma level of CD147 was determined by enzyme-linked immunosorbent assay（ ELISA）. Results STEMI group（4. 18 ± 0. 95pg /L） showed significantly higher plasma level of CD147 than control group（2. 55 ± 0. 29 pg /L）（ P 〈 0. 01）. There was a significant difference in frequencies of alleles and genotypes in 3’UTR rs8259 of CD147 with three genotypes AA,AT and TT existed（P 〈 0. 05）. Logistic regression analysis for adjusting other risk factors displayed that AT genotypes（OR= 0. 346,95% CI： 0. 210 ～ 0. 569,P 〈 0. 05） and TT genotypes（OR = 0. 107,95% CI： 0. 046 ～ 0. 251,P 〈 0. 05） can decrease the relative risk of STEMI. Allele T carriers had low onset risk for STEMI（ OR = 0. 543,95% CI： 0. 404 ～0. 730,P 〈 0. 05）,and allele A carriers had high risk for STEMI（OR = 1. 841,95% CI： 1. 370 ～ 2. 464,P 〈 0. 05）.The plasma level of CD147 was the highest in AA genotype（P 〈 0. 05）. Conclusions CD147 gene 3’UTR rs8259 allele A is probably the susceptible gene of STEMI. AA genotype can be at increased risk of STEMI due to enhancing the CD147 expression and allele T may be protective for STEMI.

关 键 词：ST段抬高型心肌梗死 CD147 基因多态性 

分 类 号：R5[医药卫生—内科学]