苯扎贝特对小鼠体内胆固醇逆转运的影响  

Effect of Benzafibrate on Reversing Cholesterol Transport in Mice in Vivo

作　　者：骆杨平[1] 董静[2] 赵水平[3] 

机构地区：[1]湖南省人民医院,湖南省长沙市410002 [2]湖南省肿瘤医院ICU,湖南省长沙市410013 [3]中南大学湘雅附二医院,湖南省长沙市410011

出　　处：《中国动脉硬化杂志》2014年第9期925-928,共4页Chinese Journal of Arteriosclerosis

摘　　要：目的观察苯扎贝特干预后小鼠血脂浓度变化,以及血清、肝脏及粪便中3H-胆固醇占经腹腔注射3H-胆固醇总量的百分比,探讨苯扎贝特对小鼠体内胆固醇逆转运的影响。方法 28只C57BL/6小鼠随机分为四组,分别给予普通饲料、不同剂量的苯扎贝特(0.1%、0.25%、0.5%)添加普通饲料喂养4周后,腹腔注射经乙酰化低密度脂蛋白(ac-LDL)及3H-胆固醇处理过的小鼠巨噬细胞悬液(每只鼠0.5 mL,细胞数达5.0×106),单独笼养24h后取血,酶法测定血脂,并测定血清、肝脏和粪便中3H-胆固醇含量(占注射总量的百分比)。结果不同剂量苯扎贝特(0.1%、0.25%、0.5%)干预后,高密度脂蛋白胆固醇(HDLC)水平升高,甘油三酯(TG)水平降低,呈剂量相关性趋势。不同剂量苯扎贝特(0.1%、0.25%、0.5%)干预后,小鼠血清3H-胆固醇含量较对照组显著增加,分别增加100%、131%、110%;小鼠肝脏3H-胆固醇含量较对照组显著增加,分别增加86.4%、52.3%、51.6%;小鼠粪便3H-胆固醇含量较对照组显著增加,分别增加110%、140%、160%。结论苯扎贝特剂量依赖性增加HDLC,降低TG。苯扎贝特能促进体内胆固醇逆转运,加速胆固醇由粪便清除,利于动脉粥样硬化的防治。Aim To investigate the effect of bezafibrate on reversing cholesterol transport from macrophage to feces in vivo,serum lipid profiles of mice were tested after bezafibrate administration. The3H-cholesterol contents in serum,liver and feces in mice were measured to explore the effect of bezafibrate on reversing cholesterol transport from macrophage to feces in vivo. Methods 28 male C57 BL /6 mice were randomly divided into four groups and treated with ordinary diet or plus different dosage（0. 1%,0. 25% and 0. 5%） of bezafibrate for 4 weeks,then these mice were injected intraperitoneally with3H-cholesterol-labeled and cholesterol-loaded RAW264. 7 macrophages（0. 5 mL /mice,the amount of cells achieve 5. 0 × 106）. Serum lipid profiles were determined by enzymatic method. Serum and liver tissues were harvested at 24 h,feces were collected. And all samples were analyzed for the appearance of3H-tracer（as the percentage of the total injected counts）. Results Bezafibrate（0. 1%,0. 25% and 0. 5%） dose-dependently and increased the level of high density lipoprotein cholesterol（HDLC） and reduced the level of triglyceride（TG）. After 24 hours of being intrapentoneally injected,the serum3H-cholesterol levels in the mice treated with different dosage of bezafibrate（0. 1%,0. 25% and 0. 5%） were significantly higher by 100%,131% and 110% compared with those in the control（P 〈 0. 05）,the liver3H-cholesterol levels in the mice treated with different dosage of bezafibrate（0. 1%,0. 25% and 0. 5%） were significantly higher 86. 4%,52. 3%,51. 6% compared with those in the control（P 〈 0. 05）,the feces3H-cholesterol levels in the mice treated with different dosage of bezafibrate（ 0. 1%,0. 25% and 0. 5%） were significantly higher by110%,140% and 160% compared with those in the control（P 〈 0. 05）. Conclusions Bezafibrate significantly and dose-dependently reduces the level of TG and increases the level of HDLC,bezafibrate promotes cholesterol reverse transport in vivo,accelerates clearanc

关 键 词：苯扎贝特 胆固醇逆转运 动脉粥样硬化 

分 类 号：R96[医药卫生—药理学]