苗药生仙汤对马尾损伤大鼠骶髓脑源性神经营养因子蛋白表达干预的研究  

Study on interference of miao medicine sheng xian decoction on protein expression of brain-derived neurotrophic factor in the sacral spinal cord of rats with cauda equina injury

作　　者：张磊[1] 熊屹[2] 王泽兰[3] 

机构地区：[1]贵阳中医学院骨伤系,贵阳医学硕士研究生550002 [2]贵阳中医学院第二附属医院骨二科,贵阳550003 [3]贵阳中医学院第二附属医院肾内科,贵阳550003

出　　处：《医学研究生学报》2014年第10期1042-1046,共5页Journal of Medical Postgraduates

基　　金：贵州省科技计划课题(黔科合中药字[2012]LKZ7006号)

摘　　要：目的脑源性神经营养因子(brain derived neurophic factor,BDNF)在马尾损伤修复中发挥了重要作用。文中拟观察苗药生仙汤对马尾神经损伤大鼠骶髓结构改变及BDNF表达的影响。方法采用神经夹持损伤的方式复制大鼠马尾神经损伤模型,按随机数字表法将大鼠分为正常对照组、空白对照组、苗药组和甲钴胺组,每组15只,各组于造模后第1天、第7天和第14天各处死5只大鼠。通过大鼠脊髓损伤功能(Basso-Beattie-Bresnahan,BBB)评分观察神经功能的恢复情况;HE染色观察大鼠骶髓结构改变;免疫组化染色观察大鼠骶髓内BDNF蛋白表达情况。结果 BBB评分:造模后第1天空白对照组评分、苗药组评分和甲钴胺组评分差异无统计学意义(P>0.05);造模后第7、14天,苗药组评分(17.0±1.00、19.60±0.55)和甲钴胺组评分(16.2±0.84、18.80±0.84)明显高于空白对照组(14.00±0.70、16.40±0.55),差异有统计学意义(P<0.05),苗药组和甲钴胺组评分值差异无统计学意义(P>0.05);空白对照组、苗药组和甲钴胺组造模后评分值随时间均呈上升趋势,组内两两比较的差异有统计学意义(P<0.05)。HE染色:与空白对照组比较,苗药组和甲钴胺组大鼠骶髓破坏较轻,胶质细胞增生也明显改善,正常神经元数量较多。免疫组化染色:造模后第1天,空白对照组MOD值(0.152±0.007)、苗药组MOD值(0.156±0.008)和甲钴胺组MOD值(0.162±0.008)均明显高于正常对照组(0.130±0.006),差异有统计学意义(P<0.05),其中,甲钴胺组MOD值明显高于苗药组和空白对照组,差异有统计学意义(P<0.05),造模后第7、14天,苗药组MOD值(0.205±0.009、0.183±0.008)和甲钴胺组MOD值(0.217±0.033、0.187±0.007)明显高于空白对照组(0.181±0.007、0.161±0.014),差异有统计学意义(P<0.05),苗药组和甲钴胺组之间MOD值差异无统计学意义(P>0.05)。空白对照组、苗药组和甲钴胺组MOD值随时间推移均呈先升后降趋势;苗药组和甲Objective Brain -derived neurophic factor （ BDNF） has played an important role in the repair of cauda equina Injury.The aim of this article was to observe the influences of Miao medicine Sheng Xian Decoction on structural change and BDNF protein expression in the sacral spinal cord of rats with cauda equina injury . Methods The rat model of cauda equina injury was es-tablished by using nerve clamping .Rats were randomly divided into the following groups：normal group , control group , Miao medicine group and mecobalamin group , 15 rats in each group .According to the death time after modeling , the rats in each group were subdivi-ded into 1d group, 7d group and 14d group, 5 in each subgroup.The nerve function recovery of rats in every group was observed through Basso , Beattie ＆ Bresnahan locomotor rating score （ BBB score ） .The structural change of sacral spinal cord was observed（11.40 ±1.14） and the mecobalamin group （11.40 ±0.89） （P〉0.05）.On the 7th, 14th day after modeling, the scores of the Miao medicine group （17.0 ±1.00, 19.60 ±0.55） and the mecobalamin group （16.2 ±0.84, 18.80 ±0.84） were much higher than the control group （14.00 ±0.70, 16.40 ±0.55） （P0.05）.The scores of the control group , the Miao medicine group and the mecobalamin group showed an increasing tendency with time, and the difference of scores among the groups are of statistical significance （P0.05）.On the 7th and 14th day after modeling, the MOD values of the Miao medicine group （0.205 ±0.009, 0.183 ±0.008） and the mecobalamin group （0.217 ±0.033, 0.187 ±0.007） were much higher than that of the control group （0.181 ±0.007, 0.161 ±0.014）, which was of significant difference （P0.05）.The MOD values of the control group , the Miao medicine group and the mecobalamin group showed a tendency of increase followed by decrease with time .The MOD values of 3 subgroups in the Miao medicine group and the mecobalamin group had no statistical significance （P〉0.05）.The MOD v

关 键 词：苗药生仙汤 甲钴胺 马尾神经损伤 脑源性神经营养因子 

分 类 号：R971[医药卫生—药品]