伏马毒素对细胞毒性作用的研究  

Study on the toxicity of fumonisin to BHK-cell

作　　者：韩薇[1] 白冰[1] 林淼[1] 倪妙娟 赵志辉[1] 

机构地区：[1]上海市农业科学院农产品质量标准与检测技术研究所,上海201403

出　　处：《上海农业学报》2014年第6期107-111,共5页Acta Agriculturae Shanghai

基　　金：上海市科委项目"热带水果电子束辐照加工关键技术及食品安全相关技术标准研究"(11dz0502800);上海市科委项目"食用农产品非法添加物及残留检测技术标准研究"(12dz0502800)

摘　　要：研究了伏马毒素(Fumonisin,Fu)对叙利亚仓鼠肾细胞(BHK-cell)的毒性作用。结果表明:随着Fu浓度递增,BHK细胞的活力下降至10%;Fu对BHK细胞DNA的损伤作用同样明显,40μg/mL剂量组的细胞拖尾率是正常对照组的80倍。40μg/mL Fu剂量组24 h后检测到特征性的凋亡梯形条带,正常对照组则未检测到。40μg/mL Fu剂量组24 h内细胞SOD活力降低至正常对照组的20%,培养液中MDA含量较正常对照组和4%乙醇对照组提高了7倍。The toxic effect of Fumonisin（Fu） on BHK-cell were studied.The results showed that with the increasing of Fu concentration,activity of BHK cell decreased to 10%;there were also obviously damage effects of Fu on DNA of BHK cells,the cells＇ tailing rate of 40 μg/mL dose group was 80 times of control group;the characteristic apoptotic ladders of 40 μg/mL Fu dose group were detected after 24 h,but the control group was not.Cell SOD activity of 40 μg/mL Fu dose group decreased to20%of control group,and the MDA content in culture medium increased 7 times compared with the control group.

关 键 词：伏马毒素 毒性作用 BHK细胞 

分 类 号：S816.3[农业科学—饲料科学]