棉花耐盐相关基因GhLIMa的克隆与表达分析  

Clone and Expression of Salt-Related Gene GhLIMa in Cotton

作　　者：王永强[1,2] 何晓亮[3] 刘建光[1] 杜淑凤[1] 赵俊丽[1] 赵贵元[1] 张寒霜[1] 

机构地区：[1]河北省农林科学院棉花研究所国家棉花改良中心河北分中心,农业部黄淮海半干旱区棉花生物学与遗传育种重点实验室,石家庄050051 [2]中国农业科学院棉花研究所棉花生物学国家重点实验室,河南安阳455000 [3]河北科技大学生物科学与工程学院,石家庄050018

出　　处：《西北植物学报》2014年第12期2382-2387,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：棉花生物学国家重点实验室开放课题(CB2013A17)

摘　　要：LIM结构蛋白是真核生物中一类重要的转录因子,参与基因转录、细胞骨架建成和信号传导等许多发育调控过程。该研究利用RT-PCR方法从棉花陆地棉耐盐材料H15中克隆得到了1个新的转录因子GhLIMa(GenBank登录号为KF601208)。GhLIMa基因包含一个627bp的开放阅读框,编码208个氨基酸残基,分子量为23.2kD。生物信息学分析发现,GhLIMa基因含有2个完整的LIM结构域,GhLIMa与可可、蓖麻、欧洲大叶杨中该蛋白的亲缘关系最近,相似性在78%以上。实时定量PCR分析显示,盐诱导后在棉花耐盐材料‘H15’的根和叶中GhLIMa基因表达量均比敏盐材料‘中棉所12’高,而且响应时间早,说明GhLIMa基因与棉花耐盐性密切相关,推测GhLIMa基因在棉花响应盐胁迫过程中具有重要作用。LIM domain proteins are important members of transcription factors in eukaryotes, which play important roles in the regulation of growth and development including gene transcription, organizing cytoskeleton and signal transduction. The full-length cDNA of GhLIMa was obtained by electronic cloning and RT-PCR method（The accession No. of Genbank is DQ672342） from salt resistant cotton materials ＇H15＇. It includes 627 bp open reading frame and encodes a protein about 23.2 kD which is composed of 208 amino acids. Bioinformatics analysis revealed that GhbIMa contains two full LIM domain structures. The amino acid sequences of LIM from cotton and other species were selected and analyzed by multi-se- quence alignment. The results showed that they have high similarity （〉78%） with those of Ricinus com- munis, Theobroma cacao, Populus trichocarpa. The expression of GhLIMa from leaves and roots of salt tolerance and salt sensitive cotton material was detected by quantitative real-time RT-PCR. The results showed that GhLIMa gene can be induced by salt in leaves and roots. It suggested that GhLIMa plays an important role in cotton response to salt stress.

关 键 词：棉花 耐盐 LIM 表达模式 

分 类 号：Q785[生物学—分子生物学] Q789