毛蕊异黄酮在金电极上的电化学行为及其与DNA相互作用的光谱与电化学研究  

Electrochemical behaviors of calycosin at gold electrode and its interaction with DNA characterized by electrochemical and spectroscopic methods

作　　者：孙莉[1] 王学亮[2] 刘哲[1] 王朝霞[2] 郁章玉[1,2] 

机构地区：[1]曲阜师范大学化学与化工学院,曲阜273165 [2]菏泽学院化学与化工系,菏泽274015

出　　处：《分析试验室》2015年第2期125-129,共5页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(21105023);山东省自然科学基金(BS2013HZ027;ZR2009BM003)项目资助

摘　　要：采用光谱法和电化学方法研究了毛蕊异黄酮（CYS）与DNA的作用。CYS与DNA作用后的紫外-可见吸收光谱,表现出一定的减色效应,其荧光光谱则表现出增色效应,表明两者能够发生相互作用。电化学研究表明在p H 4.4的HAc-Na Ac缓冲溶液中,CYS在金电极上有一对氧化还原峰,在0.35-7.04μmol/L和7.04-70.36μmol/L的浓度范围内,CYS的氧化峰电流与其浓度呈良好的线性关系。CYS与DNA作用后,CYS的氧化峰电流显著减小,峰电位发生正移。根据DNA加入前后,峰电流及峰电位的变化,计算了CYS与DNA相互作用前后的动力学参数。The interactions of calycosin（ CYS） with DNA were investigated by electrochemical methods coupled with different spectroscopic techniques. According to the spectral measurements,with the increasing amount of DNA added to calycosin,hypochromic and bathochromic shift in the maximum absorption bands of CYS in UVVis as well as the hyperchromic in emission intensity in fluorescence studies were observed,which demonstrate the interaction between CYS and DNA. The gold electrode was used for electrochemical studies of CYS-DNA interaction in acetate buffer at p H 4. 4. CYS shows a well-defined cyclic voltammetric wave. The anodic peak current is proportional to CYS concentration and can be used to determine CYS concentration. In the presence of DNA,the peak current of CYS decreased remarkably and the peak potential shifted positively. Based on the electrochemical results,some electrochemical parameters were also calculated.

关 键 词：毛蕊异黄酮 DNA 相互作用 光谱法 电化学 

分 类 号：O657.1[理学—分析化学] O657.3[理学—化学]