马兜铃酸肾损害和三七总皂苷保护作用分子机制的蛋白组学研究  

Preliminary Study on the Molecular Mechanisms of Aristolochic Acid- Induced Nephrotoxicity and Panax notoginseng Saponins Protection Using SELDI Combined with Protein Chips

作　　者：杨玉芳[1] 苏丽娜[2] 刘华钢[3] 席加喜[4] 

机构地区：[1]广西医科大学第一附属医院,广西南宁530021 [2]广西南宁市第二人民医院,广西南宁530031 [3]广西医科大学药学院,广西南宁530021 [4]广西壮族自治区人民医院,广西南宁530021

出　　处：《时珍国医国药》2015年第1期21-24,共4页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.30850010;No.81260598)

摘　　要：目的从蛋白质组学初步探讨马兜铃酸肾损害以及三七总皂苷(PNS)保护作用的分子机制。方法建立慢性马兜铃酸肾损害的大鼠模型,用PNS干预,采用表面增强激光解析电离飞行时间质谱(SELDI-TOF-MS)结合CM10芯片技术,筛选马兜铃酸模型组分别和空白对照组、PNS治疗组、泼尼松对照组之间大鼠肾组织的差异表达蛋白质。结果马兜铃酸组血清Scr、BUN和尿NAG显著高于空白对照组,PNS可使其显著降低。马兜铃酸组与空白对照组之间的肾组织差异表达蛋白质12个,马兜铃酸组分别与PNS组、泼尼松组之间的差异表达蛋白质分别有9个、31个。在这3组差异表达蛋白质中存在7个共同的差异表达蛋白质,它们在马兜铃酸组较空白对照组上调或下调,PNS和/或泼尼松可使其回调,甚至回调到接近空白对照组的水平。结论这些差异表达蛋白质尤其是共同的差异表达蛋白质可能与马兜铃酸肾毒性以及PNS的保护作用有关,进一步对其进行质谱鉴定和功能研究,将有助于探讨马兜铃酸肾损害以及PNS保护作用的分子机制。Objective To preliminary study the molecular mechanisms of aristolochic acid - induced nephrotoxicity and Panax Notoginseng Saponins（PNS） protective effect from the perspective of proteomics. Methods Rat model of chronic aristolochic acid - induced renal damage was established, and intervented with PNS, screening differentially expressed proteins of renal tissue be- tween aristolochic acid group, PNS therapy group, blank control group and prednisone group using SELDI - TOF - MS combined CM10 chip technology. Results Aristolochic acid group had significantly higher serum levels of Scr, BUN, and urinary NAG than blank control group, PNS could reduce them obviously. There were 12 differentially expressed proteins of renal tissue between aristolochic acid group and blank control group, and 9 differentially expressed proteins between aristolochic acid group and PNS group, 31 differentially expressed proteins between prednisone group and aristolochic acid group. There were 7 common differenti- ally expressed proteins in all groups. They upregulated or downregulated in the aristolochic acid group compared with the blank control group, while they were callbacked even close to the level of the blank control group in the prednisone group and/or PNS group. Conclusion These differentially expressed proteins especially these common differentially expressed proteins may be in- volved in aristolochic acid - induced nephrotoxicity, or participate in the protective effect of PNS. Identifying using mass spectrom- etry and functional studies on these differentially expressed proteins will help to explore the molecular mechanisms of aristolochic acid - induced nephrotoxicity and that of PNS protection against aristolochic acid - induced nephrotoxicity.

关 键 词：三七总皂苷 马兜铃酸 肾损害 差异表达蛋白质 蛋白质组学 

分 类 号：R284.2[医药卫生—中药学]