补阳还五汤抗Hcy诱导Eahy926内皮细胞凋亡的机制研究  

Study on the mechanism of the Bu Yang Huan Wu Decoction Anti Hcy-induced apoptosis of Eahy926 endothelial cells

作　　者：游宇[1] 佟阳[2] 涂秀英[2] 严雪梅[2] 刘玉晖[2] 

机构地区：[1]南昌大学第一附属医院,江西南昌330006 [2]江西中医学院药学院,江西南昌330004

出　　处：《时珍国医国药》2015年第1期229-232,共4页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.30960505;No.81160526);国家“973”项目资助课题(No.2010CB530603)

摘　　要：目的探讨补阳还五汤抑制Hcy诱导的内皮细胞凋亡与MAPK/ERK途径的关系。方法内皮细胞用Hcy(2mmol/L)作用时间为24h诱导损伤,治疗组给予补阳还五汤含药血清(5%、10%,20%),SB203580,PD98059,24 h后,采用Annexin V-FITC和PI双染法,用流式细胞术检测内皮细胞凋亡率,蛋白质印迹技术检测Caspase-3、Caspase-9,MAPK、ERK的蛋白质水平。结果 Hcy组HUVEC凋亡明显(P<0.01),与空白对照组相比Caspase-3,Caspase-9,MAPK,ERK蛋白含量明显升高,细胞凋亡率检测3个剂量的BYHW显著抑制Hcy诱导的内皮细胞凋亡,不同剂量的补阳还五汤显著降低内皮细胞中Caspase-3、Caspase-9,MAPK,ERK蛋白表达。结论补阳还五汤能显著抑制Hcy诱导的内皮细胞的凋亡,其可能的机制是调控内皮细胞中的MAPK/ERK信号通路的表达有关。Objective To study the mechanism of the BuYangHuanWu Decoction Anti Hcy - induced apoptosis of endothelial cells via caspase- 3, easpase- 9. Methods Human umbilical vein endothelial cells （HUVEC） were treatedWith Hcy（2mmmol/ L） for 24 hours ,The intervention group was adminstered BuYangHuanWu Decoction（5% , 10% ,20% ）SB203580 ,PD98059 . Af- ter 24 hours, cell apoptosis was evaluated by AnnexinV - FITC and PI and protein levels of Caspase - 3 , Caspase - 9 , MAPK, ERKwere analyzed by Western blot. Results The model group showed evident HUVEC apoptosis which had significant difference with others groups, Compared with control group, the level of Caspase - 3 increased , Endothelial cells apoptosis showed that Differ- ent doses of BuYangHuanWu Decoction could decrease the apopto, and the protein level of Caspase - 3 Caspase - 9 MAPK ERK （P 〈 0.01 ）. Conclusion BuYangHuanWu Decoction can prevent apoptosis of endothelial cell induced by Hcy via control MAPK/ ERK.

关 键 词：补阳还五汤 同型半光氨酸 内皮细胞 CASPASE-3 CASPASE-9 MAPK/ERK 

分 类 号：R285.5[医药卫生—中药学]