高速逆流色谱分离纯化雷公藤中的雷公藤红素  

Preparation of Celastrol from Tripterygium wilfordii Hook. F. by High-speed Countercurrent Chromatography

作　　者：樊希望[1,2] 梁上疆[1] 杜传荣[2] 刘俊琪[2] 王建平[1] 韦万兴[2] 

机构地区：[1]南宁市化工研究设计院,南宁530022 [2]广西大学化学化工学院,南宁530004

出　　处：《天然产物研究与开发》2015年第1期77-79,共3页Natural Product Research and Development

基　　金：广西科学与研究开发计划项目(0992003A-14);广西研究生教育创新计划项目(2008105930703M091)

摘　　要：以雷公藤植物粗提物为原料,建立了高速逆流色谱分离纯化雷公藤红素的分离纯化方法。优化了两相溶剂体系的组成及配比。优化后的分离纯化溶剂体系为正己烷-乙酸乙酯-甲醇-水,其体积之比为2∶3∶3∶2(上相为固定相,下相为流动相),实验温度为室温,主机转速为800 rpm,正向洗脱,流动相流速为2.0 m L/min。目标产物的分离时间较短、产品纯度高(97.5%)、分离过程稳定。A single-step preparative high-speed countercurrent chromatography（HSCCC） method was developed for the separation and purification of celastrol from the extract of Tripterygium wilfordii Hook. F.（TWHF）. The results showed that under the selected solvent system（n-hexane/ethyl acetate/methanol/water = 2∶ 3∶ 3∶ 2,V/V/V/V,heavy phase as mobile phase and light phase as stationary phase）,flow rate of the mobile phase（2. 0 m L/min） and clockwise rotation,the mentioned compound was effectively separated at 25. 0 ℃ in rotational speed of 800 rpm. The developed HSCCC method was quick,stable and efficient.

关 键 词：高速逆流色谱(HSCCC) 雷公藤红素 雷公藤 

分 类 号：R284.2[医药卫生—中药学]